【摘要】 根据JJF1059-1999《测量不确定度评定与表示》规范对ELISA法测定组胺的不确定度进行评价分析,分别计算各分量的不确定度,进而计算合成不确定度。取k=2(置信概率95%),当样品rA17的组胺浓度为11.47μg/mL时,移取溶剂相对不确定度为0.015μg/mL,重复性试验相对不确定度为0.0395μg/mL,曲线拟合的相对不确定度0.021μg/mL,合成相对标准不确定度为0.047μg/mL,其扩展不确定度为1.08μg/mL,建立适合ELISA法测定组胺浓度的不确定度分析。更多还原

【Abstract】 The uncertainty for determination of histamine by ELISA was studied based on "Evaluation and Expression of Uncertainty in Measurement"(JJF1059-1999).The combined uncertainty was obtained by combining all standard uncertainty,and the expanded uncertainty was calculated by using a coverage of factor k=2,giving a level of confidence of approximately 95%,then to establish the evaluation of uncertainty which is suitable to measure the concentrations of histamine by ELISA.The uncertainty for determination of transfering solution and repeatability test and curve fitting,respectively,is 0.015 μg/mL and 0.0395 μg/mL and 0.021 μg/mL.Synthesis of relative standard uncertainty is 0.047 μg/mL.更多还原