【摘要】 目的探讨血管紧张素Ⅱ(AngⅡ)和血管紧张素1-7(Ang1-7)对大鼠肝星状细胞Rho-Rock信号转导通路的影响。方法采用HSC-T6细胞株,分别给予AngⅡ、Ang1-7、AngⅡ+Ang1-7、Ang1-7+A779 10μmol/L处理,逆转录聚合酶链反应(RT-PCR)检测Rho-Rock通路中Rock2 Rho kinase2)、RhoAGTP、RhoGEF Rho Guanine Nucleotide Exchange Factors,RhoGEF)的表达。结果 AngⅡ处理组Rock2、RhoAGTP、RhoGEF mRNA的表达显著增强,Ang1-7组Rock2、RhoAGTP、RhoGEF mRNA的表达显著低于阴性对照组。结论 Ang1-7可抑制AngⅡ诱导的Rho-Rock信号通路的表达。更多还原

【Abstract】 Objective To investigate the effects of Angiotensin1-7 on Rho-Rock pathway of rat hepatic stellate cell. Methods HSC-T6 cells were treated with 10 μmol/L of AngII, Ang1-7,AngⅡ+Ang1-7, and Ang1-7+A779, respectively. RT-PCR was used to detect the expression of Rock2,RhoAGTP and RhoGEF in Ca 2+ -independent pathways mediated by Rho-kinase. Results The mRNA expression of Rock2, RhoAGTP, and RhoGEF was significantly increased after AngII treatment (P<0.01), but decreased following subsequent Ang1-7 treatment. Conclusion Ang1-7 could inhibit AngⅡ-induced activation of the Rho-Rock pathway.更多还原