【摘要】 目的研究视网膜母细胞瘤(retinoblastoma,RB)和正常视网膜组织中血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)的表达和淋巴管计数,探讨其相互关系及意义。方法收集30例RB眼球摘除手术标本和15例正常视网膜组织标本,采用Envision免疫组织化学法行VEGF-C和淋巴管染色。结果 RB组织中VEGF-C表达阳性率及淋巴管计数明显高于正常视网膜组织(P<0.01);临床分期Ⅰ期、分化型、未侵犯视神经及生存期≥2年RB病例VEGF-C表达阳性率及淋巴管计数明显低于临床分期Ⅲ期、未分化型、侵犯视神经和生存<2年病例(P<0.05或P<0.01)。VEGF-C阳性表达RB中淋巴管计数明显高于阴性病例(P<0.01)。结论 VEGF-C表达水平和淋巴管计数可能是反映RB进展、生物学行为和预后的标记物,VEGF-C可能具有促进RB组织中淋巴管生成作用。更多还原

【Abstract】 Objective To investigate the VEGF-C expression and lymph vessel counts in retinoblastoma(RB) and normal retina tissue,to study their interrelationship and clinicopathological significance.Method Envision immunohistochemistry stainings of VEGF-C and D2-40 were performed in 30 retinoblastoma and 15 normal retina tissues,which was routinely paraffin imbedded.Results The positive rate of VEGF-C expression and lymph vessel counts were higher in RB tissue than normal control(P<0.01);Samples from the clinical stage Ⅰ,differentiated type,uninvolved optic nerve and life span ≥2 year cases had higher expression of VEGF-C and higher lymph vessel counts than those from clinical stage Ⅲ,undifferentiated type,involved optic nerve and life span <2 year cases(P<0.05 or P<0.01).Lymph vessel counts in VEGF-C positive samples were much higher than negative ones.Conclusion VEGF-C expression and lymph vessel counts maybe useful markers of RB progression,biological behavior and prognosis;VEGF-C might have promotive effect on lymphangiogenesis in RB tissue.更多还原