【摘要】 背景:目前人们把目光多集中在干细胞修复脊髓损伤方面,而对于干细胞移植治疗周围神经损伤的研究却不多,尤其人羊膜上皮细胞修复治疗周围神经损伤的研究和报道更少。目的:探讨人羊膜上皮细胞对臂丛神经的修复作用。方法:应用测力神经根拉钩水平牵拉臂丛神经根的方法制备大白兔臂丛损伤模型,提取人羊膜上皮细胞并扩增培养,扫描电子显微镜下观察人羊膜组织及羊膜上皮细胞的超微结构,人羊膜上皮细胞转染绿色荧光蛋白标记并注射入臂丛神经损伤区,苏木精-伊红染色及荧光显微镜观察羊膜上皮细胞对兔臂丛神经损伤的修复作用。结果与结论:①分离得到的人羊膜上皮细胞表达上皮细胞标志物角蛋白CK19,不表达间充质细胞标志物波形蛋白,不同程度表达CD29,CD73。扫描电子显微镜观察到人羊膜上皮细胞胞体饱满,连接紧密,细胞状态良好。②人羊膜上皮细胞移植到新西兰大白兔臂丛神经损伤模型后18周,免疫荧光检测到神经损伤区可见有表达绿色荧光蛋白的羊膜上皮细胞。③术后6周实验组大白兔前爪功能开始恢复,术后12,18周实验组大白兔前爪功能得到明显改善,功能评分明显提高,对照组几乎没有恢复。以上结果可见人羊膜上皮细胞参与了臂丛神经损伤的修复。更多还原

【Abstract】 BACKGROUND: A great deal of attention has been given to stem cell transplantation for repair of spinal cord injury, while there are few studies concerning stem cell transplantation for repair of peripheral nerve injuries, especially transplantation of human amniotic epithelial cells used to repair peripheral nerve injuries. OBJECTIVE: To discuss the effects of human amniotic epithelial cells in brachial plexus nerve repairing. METHODS: We established rabbit models of brachial plexus injuries and extracted human amniotic epithelial cells for expanding culture followed by ultrastructural observation of human amniotic membrane tissue and amniotic epithelial cells under a scanning electron microscope. Then, human amniotic epithelial cells were transfected with green fluorescent protein markers and injected into the injury site. Hematoxylin-eosin staining and fluorescence microscope were employed to observe the effects of human amniotic epithelial cells in the repair of brachial plexus injuries in rabbits. RESULTS AND CONCLUSION:(1) Successfully isolated human amniotic epithelial cells expressed cytokeratin 19 rather than vimentin, and also expressed CD29 and CD73 to different extent. Scanning electron microscope observation showed human amniotic epithelial cells had filled cell bodies and were closed interconnected in good condition.(2) After 18 weeks of successful modeling, human amniotic epithelial cells labeled with green fluorescent protein were visible in the injury site under the fluorescence microscope.(3) In the experimental group, functional recovery of the rabbit forelegs was found at postoperative 6 weeks and dramatically improved at 12 and 18 weeks after surgery. Functional scores were also significantly increased in the experimental group. However, there was no improvement in the control group. These findings indicate that human amniotic epithelial cells are involvedin the repair of brachial plexus injury.更多还原