【摘要】 背景:细胞支架复合物在体外构建及成熟分化为骨组织工程骨是骨组织工程构建的必须过程,目前尚无统一方法及标准。目的:总结目前骨组织工程构建的技术与基本方法并讨论相关发展。方法:以"bone tissue engineering,cell biological scaffold,cell inoculation,seeding density,culture in vitro,bioreactor"为英文检索词,以"骨组织工程,生物支架,细胞接种,接种密度,体外培养,生物反应器"为中文检索词,检索PubMed数据库和中国期刊全文数据库1997年1月至2013年1月与骨组织工程骨体外构建和外培养相关文献,根据纳入排除标准,最终纳入44篇。结果与结论:生物支架作为骨组织工程种子细胞的载体,首要的前提是无菌,无菌状态的细胞支架复合物才能够存活。生物支架材料的灭菌有紫外线灭菌法,60Coγ射线灭菌法,体积分数75%乙醇浸泡,高压灭菌锅法,环氧乙烷灭菌等方法。60Coγ射线灭菌方法常在支架灭菌中成为较常选的方法。种子细胞的接种密度是影响种子细胞在支架上黏附生长及增殖的一个重要因素。细胞与支架材料的黏附受支架材料亲合力、细胞黏附力及重力等多方面因素的影响。目前阶段骨组织工程种子细胞接种方法分位静态接种和动态接种。每一种构建方法都各有优缺点,克服这些缺点以及形成统一的构建方法并充分应用临床是未来的发展方向。更多还原

【Abstract】 BACKGROUND: The in vitro construction, maturation and differentiation of cell scaffold complexes into tissueengineered bone is the necessary process of bone tissue engineering construction, but there are no uniform methods and standards. OBJECTIVE: To summarize the basic method and technology to build bone tissue engineering at present and to discuss the related development. METHODS: A compute-based online search was conducted on the PubMed database and CNKI database for the articles related to the in vitro construction and culture of bone tissue engineering bone from January 1997 to January 2013 with the key words of "bone tissue engineering, cell biological scaffold, cell inoculation, seeding density, culture in vitro, bioreactor" in English and Chinese. Finally, 44 articles were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION: As the carrier of bone tissue engineering seed cells, the primary prerequisite of biological scaffold is sterile, because the sterile biological scaffold can be able to survive. Sterilization of biological scaffolds includes ultraviolet sterilization,60Co γ-ray sterilization, soaking in ethanol with the volume fraction of 75%, autoclave method, and ethylene oxide sterilization.60Co γ-ray sterilization is the common method in thebiological scaffold sterilization. The inoculation density of seed cells is the key factors that influence the adhesion growth and proliferation of seed cells on the scaffolds. The adhesion between cells and scaffold materials will be affected by the affinity of scaffolds, cell adhesion and gravity, and other factors. The method for the inoculation of bone tissue engineering seed cells includes static inoculation and dynamic inoculation. Each construction method has its advantages and disadvantages. Overcomeing these disadvantages, forming a uniform construction method and fully clinical application are the direction of future development.更多还原