【摘要】 背景:Nc/Nga小鼠皮损、病理和免疫学特征等均与人类特应性皮炎相一致,作为特应性皮炎动物模型其研究价值日益显现。目前国内尚无Nc/Nga小鼠的生理生化指标等相关数据报道。目的:观察特应性皮炎动物模型Nc/Nga小鼠的生长繁殖性能以及血液生理、生化指标。方法:统计Nc/Nga小鼠1-3胎的繁殖指标数据,包括每胎产仔数、离乳率、怀孕率及胎间隔。分别称取60只1-56 d Nc/Nga仔鼠的体质量,雌雄各半,绘制其生长表格。Nc/Nga鼠眼眶采血,测定其血常规和生化值。结果与结论:Nc/Nga小鼠平均交配分娩间隔为(25.8±3.1)d,平均产仔数为(7.5±2.5)只,平均离乳率为(97.2±1.2)%、平均怀孕率为(97.0±1.4)%,1-3胎间差异无显著性意义(P>0.05)。Nc/Nga小鼠的体质量随着日龄的增长而逐渐增加,断奶后一两周内增重最为迅速,6周后体质量雌雄间差异无显著性意义(P>0.05)。同一日龄的雌雄间血常规和生化值比较,雌性小鼠红细胞、血红蛋白明显高于雄性小鼠;雄性小鼠血小板计数明显高于雌性小鼠(P<0.05);雌性小鼠三酰甘油、白蛋白水平明显高于雄性小鼠(P<0.05)。提示性别和年龄都会影响Nc/Nga小鼠的血液生理和生化值指标。更多还原

【Abstract】 BACKGROUND: The skin lesions, pathological and immunological characteristics of Nc/Nga mice are consistent with human atopic dermatitis, and as an atopic dermatitis animal model, it has great research value. But there are no reports on the physiological and biochemical parameters of Nc/Nga mice in China.OBJECTIVE: To observe the growth and reproduction and blood physiological and biochemical parameters of atopic dermatitis model of NC/Nga mice METHODS: The data of reproductive performance of NC/Nga mice from the first to the third generation was analyzed, including mean litter size, weaning rate, pregnancy rate and generation interval. The body mass of 60Nc/Nga mice with 1-56 days old was measured, 30 mice of female and male, then the growth curve was draw.The blood samples from the infraorbital vessels were collected to detect the blood physiological and biochemical parameters in the mice.RESULTS AND CONCLUSION: The mean delivery interval of Nc/Nga mice was(25.8±3.1) days, mean litter size was(7.5±2.5) mice, mean weaning rate was(97.2±1.2)%, the mean pregnancy rate was(97.0±1.4)%, and there was no significant difference among the three generations of these mice(P > 0.05). The body mass of Nc/Ngamice was increased with the time increasing of days, the body mass of the mice was maximal within 1-2 weeks post-weaning, and there was no significant difference in body mass between males and females at 6 weeks post-weaning(P > 0.05). Comparison of the blood physiological and biochemical parameters of mice at the same age between males and females showed that the levels of red blood cells and hemoglobin in female mice were significantly higher than those in the male mice; the platelet count in the male mice was significantly higher than that in the female mice(P < 0.05); the triglycerides and albumin levels in the female mice were higher than those in the male mice(P < 0.05). The results indicate that the gender and age may influence the blood physiological and biochemical parameters of Nc/Nga mice.更多还原