【摘要】 背景:间充质干细胞是可大量获取的肌腱组织工程种子细胞,但如何体外诱导成为此项技术的关键。目的:观察富血小板血浆对体外培养间充质干细胞分裂增殖和胶原产生的影响。方法:分离培养兔间充质干细胞,设立富血小板血浆高、中、低剂量组干预间充质干细胞,并设空白组做对照。结果与结论:富血小板血浆高、中、低组间充质干细胞均保持较高的增殖活性,呈快速生长,曲线上升幅度大,与空白对照组比较差异有显著性意义(P<0.05)。培养时间越长作用越明显,培养一定时间后其作用具有剂量依赖性,较高剂量的富血小板血浆对细胞的增殖作用较为明显。提示富血小板血浆能明显促进间充质干细胞的Ⅰ和Ⅲ型胶原合成,剂量越大,刺激胶原产生的作用越明显。更多还原

【Abstract】 BACKGROUND: Mesenchymal stem cells are the seed cells for tendon tissue engineering which can be obtained in large quantities, but how to induce in vitro is a key technology. OBJECTIVE: To explore the effect of platelet-rich plasma on the proliferation and collagen production of in vitro cultured mesenchymal stem cells. METHODS: The rabbit mesenchymal stem cells were separated ad cultured. The high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group were set to induce the mesenchymal stem cells, and the blank control group was set as control. RESULTS AND CONCLUSION: The proliferation of mesenchymal stem cells in the high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group was high, and in rapid growth with big increase amplitude, and there was no significant difference in the proliferation when compared with the blank control group(P < 0.05). The effect was positively correlated with the culture time, and after cultured for a certain time, the effect was in dose-dependent manner, as higher dose platelet-rich plasma had more significant effect on the proliferation of the cells. The results indicate that platelet-rich plasma can significantly promote the synthesis of collagen type Ⅰ and Ⅲ of mesenchymal stem cells, the higher the dose, the more significant the effect on the collagen.更多还原