【摘要】 背景:随着干细胞技术的发展,利用组织工程技术修复软骨损伤成为一种可能,而骨髓间充质干细胞由于其优良的特性成为研究重点。目的:通过体外培养骨髓间充质干细胞,注入兔颞下颌关节紊乱病动物模型,观察干细胞对兔颞下颌关节紊乱病的治疗效果。方法:通过体外全血贴壁法培养骨髓间充质干细胞并进行鉴定;细胞在体外扩增,诱导成软骨细胞后待用。以Ⅱ型胶原酶进行颞下颌关节腔内注射,建立颞下颌关节紊乱病动物模型,关节腔内注射诱导后成软骨细胞设为实验组,对照组注射未进行诱导的细胞进行比较,通过观察动物咀嚼和组织切片观察治疗效果。结果与结论:实验分离的细胞7-14d可见少量集落形成,20d时观察见细胞基本铺满瓶底。经stro-1+、CD44+流式细胞及免疫组化测定细胞表达间充质干细胞特性;细胞在诱导成软骨细胞后Ⅱ型胶原免疫组化染色强阳性。兔颞下颌关节注射胶原酶可在2周时出现偏侧咀嚼症状,骨髓间充质干细胞诱导的成软骨细胞关节腔注入动物模型后,实验组明显弱于对照组。组织切片显示诱导的成软骨细胞可促进关节损伤的修复,软骨及胶原生成多于对照组。说明骨髓间充质诱导的成软骨细胞关节腔注入后可促进兔颞下颌关节骨关节病愈合。更多还原

【Abstract】 BACKGROUND: With the development of stem cell technology, it is possible to repair cartilage injury using tissue engineering technology, and bone marrow mesenchymal stem cells have become the research focus because of their advantages. OBJECTIVE: To observe the treatment effect of stem cells on rabbit temporomandibular joint disorders through injection of bone marrow mesenchymal stem cells cultured in vitro into a rabbit model of temporomandibular joint disorder. METHODS: The bone marrow mesenchymal stem cells were cultured and identified with in vitro whole blood adherent method. Then the cells were amplified in vitro and induced to differentiate into chondrocytes for using. Intraarticular injection of type Ⅱ collagenase was performed to establish the animal model of temporomandibular joint disorder. In the experimental group, intraarticular injection of induced chondrocytes was performed; and in the control group, the rabbits were injected with un-induced cells. The treatment effect was compared between two groups through observing the chewing and tissue sections of the animals. RESULTS AND CONCLUSION: A small amount of colony formation could be seen in the separated cells at 7-14 days; and at 20 days, the cells covered the bottom of the bottle basically. The stro-1+ and CD44+ flow cytometry and immunohistochemical determination showed the cells had the characteristics of stem cells; strong positive type Ⅱ collagen immunohistochemical staining of cells could be seen after induced into chondrocytes. Symptoms of unilateral chewing could be observed at 2 weeks after intraarticular injection of type Ⅱ collagenase. After intraarticular injection of bone marrow mesenchymal stem cells induced chondrocytes into the animal models, the unilateral chewing in the experimental group was weaker than that in the control group. Tissue sections showed that the induced chondrocytes could promote the repair of joint injury, and the cartilage and collagen generation in the experimental group was more than that in the control group. Intraarticular injection of bone marrow mesenchymal stem cells induced chondrocytes can promote the healing of rabbit temporomandibular joint osteoarthrosis.更多还原