肿瘤干细胞(cancer stem cells,CSC)是指一类具有自我更新(self-renewal)能力的未分化细胞。在肺腺癌中,CSC的自我更新调控机制类似胚胎干细胞,即高表达OCT4、Nanog和Sox2多潜能基因,但目前对其表型特征尚存争议。该文采用成球试验(sphere-forming assay)从SPC-A1细胞株中富集CSC后进行分子表型分析。结果显示,此类肺球体细胞(pulmospheres)同时表达肺脏近端和远端呼吸上皮的多个谱系(lineage)标志,包括纤毛柱状细胞标志FoxJ1、非纤毛柱状细胞(即Clara细胞)标志CCSP、肺神经内分泌细胞标志GRP、II型肺泡细胞标志SP-C及其转录调控因子TTF-1。这些肺球体细胞也能够被3株小细胞肺癌特异性单抗(2F7、4B3和E6)所识别。通过基因沉默技术使得肺球体细胞中OCT4表达转阴后,上述标志(除E6外)均消失。研究结果揭示,肺癌CSC具有肺脏呼吸上皮多潜能细胞的表型特征。此外,初步研究结果发现,中药冬虫夏草(Hirsutella Hepialid of Cordyceps Sinensis)的被毛孢菌丝体中含有新颖抗癌成分,能够显...
【英文摘要】
Cancer stem cells(CSC) represent a subtype of undifferentiated cells that have the ability to self-renew. It has been recognized that for the CSC of lung adenocarcinoma, the regulatory mechanisms of selfrenewal are identical to the embryonic stem cells, that is, they over-express OCT4, Nanog and Sox2 pluripotent genes. However, the phenotypic characteristics of these malignant stem cells remained disputes currently. With the sphere-forming assay, we herein enriched the CSC from SPC-A1 cell line and thereby,...
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