Study of intricate transcription factor regulatory networks is main issue of illustrating mechanism of embryonic development and differentiation.ChIP-Seq combines chromatin immunoprecipitation(ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.ChIP-Seq is widely used to analyze regulatory networks of transcription factors.This paper provides a brief overview of the ChIP-Seq method and application in transcription factors regulation during embyonic developme...