【摘要】 盘基网柄菌细胞黏附分子DdCAD-1是在细胞发育过程中最先表达的黏附分子,为了研究DdCAD-1在盘基网柄菌细胞发育中的作用,将cadA基因的突变株cadA-细胞用中性红染料染色,发育成的蛞蝓体显示cadA-细胞的前柄细胞/前孢子细胞的分化出现明显的障碍,外源表达的重组蛋白His6-DdCAD-1与cadA-细胞作用一段时间后,这种现象得到了改善。另外,cadA-细胞的孢子产率也有所降低,外源重组蛋白也可以拯救该表现型。表达DdCAD-1的细胞与cadA-细胞共同发育所形成的嵌合体显示表达DdCAD-1的细胞占据在拔顶期结构的顶端及尾部,而这些结构都在非孢子区,最终会死亡。提示DdCAD-1对于细胞分化及细胞命运决定有重要意义。更多还原

【Abstract】 Cell adhesion molecule cadA/DdCAD-1 is the first expressed adhesion molecule during the development of Dictyostelium discoideum,to investigate the role of DdCAD-1 in D.discoideum development,cadA mutant strain cadA– cells were colored by Natural Red,slug structure show obviously aberrant cell differentiation.Exogenous recombinant protein His6-DdCAD-1 rescued this phenomenon.Additionally,recombinant protein can also rescue the decreasing spore yield of cadA–cells.When DdCAD-1 expressed cells were mixed with cadA–cells,DdCAD-1 expressed cells occupied the tips and posterior region of the chimeric culmination structures,which do not belong to spore area and would die eventually.All these results suggest that DdCAD-1 plays a crucial role in cell differentiation and cell fate determination.更多还原