【摘要】 目的研究猪纤维蛋白原的免疫原性和免疫效果,为该药物安全评价和进一步优化提供实验依据。方法建立生物素-亲和素系统改良的ABC-ELISA检测抗体;新西兰白兔创伤试验考察血清抗体效价及其动态变化;免疫器官质量及脏器指数分析、噻唑蓝(MTT)比色法检测脾淋巴细胞增殖、氯化硝基四氮唑蓝(NTB)还原法检测中性粒细胞吞噬能力,揭示猪纤维蛋白原对小鼠免疫系统的影响。结果 1周后新西兰白兔血清即可检测出抗体,且第2周抗体含量达到峰值后下降至较稳定水平,抗体效价也呈现相同的变化趋势;小鼠免疫器官质量及脏器指数均未发生明显改变;与对照组相比,各实验组小鼠淋巴细胞增殖指数无明显差异;实验组小鼠中性粒细胞吞噬能力显著性增强。结论猪纤维蛋白原刺激新西兰白兔机体后产生体液免疫应答,但在小鼠体内未表现出特异性免疫反应。更多还原

【Abstract】 Objective To investigate the immunogenicity and immune effect of porcine fibrinogen and provide experimental evidence for safety evaluation and further optimization of the drug.Methods An improved ABC-ELISA system of biotin and conjugated streptomyces avidin was used for detecting antibodies.The serum antibody titer and its dynamics were studied by means of the injury experiment in New Zealand rabbits.Effects of porcine fibrinogen on immune system of mice were evaluated by analyzing the weight gain and indexes of immune organs,the proliferation of spleen lymphocytes by thiazole blue(MTT) chromatometry and the neutrophil phagocytosis by nitro-tetrazolium chloride blue(NTB) reduction method.Results The antibodies could be detected in New Zealand white rabbit serum 1 week after immunization,and antibody levels decreased to a more stable level after it peaked at the second week,at the same time,the antibody titer also showed the same trend.The weight and indexes of immune organs were not changed significantly in mice.Compared with the control group,the proliferation rate of lymphocytes in each experimental group didn’t show statistical difference,but neutrophil phagocytosis of the experimental groups was enhanced significantly.Conclusion The humoral immune response was induced in New Zealand rabbits after stimulated with porcine fibrinogen,but no specific immune response was found in mice.更多还原