【摘要】 目的探讨复方三七饮(CPNG)对博莱霉素(BLM)致大鼠肺纤维化的干预作用及其机制。方法雄性SD大鼠60只,随机分为6组:正常组、模型组、醋酸泼尼松组、CPNG大、中、小剂量组[CPNG(H、M、L)],每组10只。经气管内注入BLM(5 mg/kg)制备大鼠肺纤维化模型。造模第2天,治疗组分别灌服醋酸泼尼松3.33 mg/(kg·d)、CPNG大、中、小剂量(100、50、25)mg/(kg·d),正常组和模型组大鼠灌服等体积的蒸馏水,1次/d。28 d后处死大鼠,HE染色和Masson染色进行肺组织病理学观察;碱水法测定肺组织中羟脯氨酸(HYP)含量;Western blot法检测TGF-β1、Smad2、Smad7蛋白的表达水平。结果与模型组大鼠比较,肺组织的病理组织学HE和Masson染色显示CPNG能明显减轻大鼠肺泡炎和肺纤维化程度(P<0.01);CPNG各剂量组大鼠肺组织中HYP降低(P<0.01);CPNG各剂量组TGF-β1、Smad2蛋白的表达水平降低(P<0.01),Smad7蛋白表达水平升高(P<0.01)。结论复方三七饮对博莱霉素致大鼠肺纤维化具有一定的防治作用,可能与调节TGF-β1/Smads信号通路有关。更多还原

【Abstract】 Objective To investigate the intervention effects and mechanisms of the compound panax notoginsenoside granules(CPNG) on bleomycin-induced pulmonary fibrosis in rats.Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups,including normal control group,model group,prednisolone group,and CPNG(high,middle and low-dose) groups.Each group included 10 Sprague-Dawley rats.Except the control group,pulmonary fibrosis model rats were injected with bleomycin(5 mg/kg) via trachea.On the second day of the bleomycin injection,the rats in each treatment group were given prednisolone(3.33 mg/kg) and corresponding doses of CPNG(100,50,25 mg/kg) by nasogastric feeding daily;the normal control group and the model group were given the equal volume distilled water daily.Twenty-eight days later,all rats were executed and the lung tissues were subjected to the histopathological examination via HE and Masson staining.The hydroxyproline(HYP) in lung tissues was assessed by alkaline hydrolysis.The expressions of TGFβ1,Smad2,and Smad7 proteins were analyzed by Western blotting.Results Compared with the model group,the histopathological results revealed that CPNG dramatically decreased the severity of lung inflammation and fibrosis in rats(P<0.01).Compared with the control group,the HYP in lung tissues was reduced in all of the CPNG groups(P<0.01). Moreover,CPNG inhibited the expressions of TGF-β1 or Smad2 proteins(P<0.01),but enhanced the expression of Smad7 protein in the rat lung tissues(P<0.01).Conclusion CPNG has the prevention and treatment effect on the bleomycininduced pulmonary fibrosis in rats,and it might be attributed to the regulation on the TGF-pi/Smads signaling pathway.更多还原