【摘要】 目的研究白藜芦醇对小鼠CD4+T细胞活化的影响。方法将BALB/c鼠(SPF,雌性,9～10周龄)脾脏单个核细胞与不同浓度(0、10、20、40 mmol/L)的白藜芦醇共同孵育30 min后,分别采用刀豆蛋白A(ConA)、抗CD3抗体、抗CD3/抗CD28抗体刺激继续培养;分别共培养6 h、36 h后收集细胞采用单克隆抗体抗CD4-PerCP和抗CD69-FITC或抗CD71-FITC染色后,流式细胞术检测CD4+T细胞CD69、CD71的表达。结果白藜芦醇(≥10 mmol/L)可显著抑制抗CD3抗体引起的CD4+T淋巴细胞CD69表达(P<0.01),其抑制作用呈剂量依赖关系。白藜芦醇(≥20 mmol/L)可显著抑制由ConA、抗CD3/CD28抗体引起的CD4+T淋巴细胞CD69表达(P<0.05),其抑制作用呈剂量依赖关系。白藜芦醇(≥10 mmol/L)可显著抑制ConA引起的CD4+T淋巴细胞CD71表达(P<0.05),其抑制作用呈剂量依赖关系。白藜芦醇(≥20 mmol/L)可显著抑制由抗CD3抗体、抗CD3/抗CD28抗体引起的CD4+T淋巴细胞CD71表达(P<0.05),其抑制作用呈剂量依赖关系。结论白藜芦醇具有抑制小鼠CD4+T细胞活化的作用,其抑制作用呈剂量依赖关系。更多还原

【Abstract】 Objective To study the effect of resveratrol on murine CD4+ T lymphocyte activation.Methods Splenic mononuclear cells(SMCs) from specific pathogen free(SPF) BALB /c mice(female,aged 9-10 weeks) were cultured with resveratrol(0,10,20,and 40 mmol /L) for 30 min in vitro,and then were activated with ConA,anti-CD3 or anti-CD3 /antiCD28.At 6 h(for CD69) and 36 h(for CD71),cells were harvested for being stained with anti-CD4-PerCP and anti-CD69FITC,or anti-CD4-PerCP and anti-CD71-FITC,and the expressions of CD69 and CD71 were detected respectively using flow cytometry.Results Resveratrol(≥10 mmol /L) significantly decreased CD69 levels on CD4+T lymphocytes activated with anti-CD3 in a dose-dependent manner(P < 0.01).In addition,resveratrol(≥20 mmol /L) decreased CD69 levels on CD4+ T lymphocytes activated with ConA,and anti-CD3 /anti-CD28 in a dose-dependent manner(P < 0.05).When concentrations ≥10 mmol/L,resveratrol depressed CD71 expression on ConA stimulated CD4+T lymphocytes(P < 0.05),and resveratrol(≥20 mmol/L) decreased CD71 levels on anti-CD3 and anti-CD3/anti-CD28 stimulated CD4+T lymphocytes(P < 0.05),all in a dose-dependent manner.Conclusion Resveratrol inhibits the activation of murine CD4+ T lymphocytes in a dosedependent manner.更多还原