【摘要】 目的探讨中国北方汉族男子脑型肌酸激酶(CKB)基因多态性与耐力训练对心功能影响的关联性。方法102名无训练史的健康男子进行18周有氧耐力训练,训练前后测定左心室结构与功能相关指标;用PCR-RFLP法分析CKB基因启动子上rs3759582和rs3759584两单核苷酸多态性。结果二个多态位点均符合H-W平衡,不存在强的连锁关系。训练前左心结构和功能各指标在CKB两基因位点各组均无显著性差异(P>0.05);训练后rs3759582位点AC组△100WEDD、△150WEDD、△100WSV、△150WSV、△150WSV和△reT增加率显著大于AA组(P<0.05)。△CO和△COI在AC组下降,AA组增加;△150WCO和△150WCOI在AC组上升,AA组下降,且在基因型间均有显著性差异(P<0.05)。训练后各指标变化在rs3759584位点各基因型间均无显著性差异(P>0.05)。结论 CKB基因的rs3759584多态性不是个体心功能耐力训练敏感性的基因标记;rs3759582多态性可能与个体心功能对耐力训练敏感性相关,AC型可能更有利于个体心功能对耐力训练的改善。更多还原

【Abstract】 Objective To explore the association between CKB(creatine kinase,brain) gene polymorphisms and the phenotypes of structure and function of left ventricle after endurance training in men of Han nationality in northern China.Methods A group of 102 healthy male in northern China was recruited to undergo aerobic training program for 18 weeks.The rs3759582 and rs3759584 polymorphisms of CKB gene were analyzed by PCR-RFLP.Results Genotype distributions were in Hardy–Weinberg equilibrium at both loci,and no strong linkage disequilibrium was observed between rs3759584 and rs3759582.No significant differences were observed in the baseline structure and function index of left ventricle among groups at both loci before training.The △100WEDD、△150WEDD、△100WSV、△150WSV、△150WSV and △reT after training in those carrying AC were significantly more than those carrying AA at rs3759582(P<0.05).The △CO and △COI in AC were decreased,but increased in AA;the △150WCO and △150WCOI were increased in AC,but decreased in AA at rs3759582.Furthermore,significant differences were observed in all △CO、△COI、△150WCO and △150WCOI between AC and AA at rs3759582(P<0.05).But no significant differences in the structure and function index changes of left ventricle were observed among groups at rs3759584 after training(P>0.05).Conclusions The rs3759584 was not a gene marker in predicting individual endurance trainability on function of left ventricle.The rs3759582 polymorphism might contribute to individual function of left ventricle response to endurance training.更多还原