【摘要】 目的:观察补体抑制剂眼镜蛇毒因子(CVF)对脓毒症大鼠心肌组织的保护作用。方法:144只清洁级雄性SD大鼠(体质量250～300 g)被随机分为大鼠假手术组(A组)、脓毒症组(B组)、CVF组(C组)3组,每组48只。A组仅开腹翻动盲肠后关腹并复苏,B组和C组均行经典盲肠结扎穿孔(CLP)制作成脓毒症模型。C组于术前24 h给药,A组和B组则给予等量0.9%氯化钠溶液;各组按照标本采集点的不同又分为术后2、8、16、24 h时间点检测血清补体C3水平、心肌组织髓过氧化物酶(MPO)的变化及观察各组大鼠心肌组织病理学改变。结果:①B组术后血清补体C3浓度最高,C组最低(P<0.05),且一直维持较低水平;②在8、16和24 h时C组MPO活性较B组显著下降(P<0.05),其中以术后16 h最为明显;③C组不同时点心肌损伤均较B组有所减轻,三组不同时间点的差异以术后16 h最为明显。结论:CVF可以明显减少血清补体C3及MPO水平,并有效减轻脓毒症大鼠的心肌损伤,显示出对心肌组织良好的保护作用。更多还原

【Abstract】 Objective: To observe the protective effects on myocardial tissues by inhibition of complement activation of cobra venom factor(CVF) in septic rats.Methods: One hundred and forty-four SD rats(male,250~300 g) were randomly divided into three groups(each n=48): group A(sham group): abdomen was opened and closed later but without cecal ligation and puncture(CLP);group B(sepsis group): sepsis was induced by CLP;group C(CVF pretreatment group): sepsis was induced by CLP after pretreatment with CVF.At 24 hours before the operation,rats were given a dose of 50 μg/kg CVF in group C or an equal volume of saline solution in both groups A and B.To measure the levels of both complement C3 in serum and MPO,meanwhile the histopathological changes of myocardial tissues were observed.Results: There were significant differences in plasma complement C3 among the three groups;group B after operation was higher than group A and group C(P < 0.05),the level in group C was the lowest(P < 0.05) and remained low level in all time points.The MPO levels of myocardial tissues in group A remained low in all time points.However,the levels in group B and group C raised quickly after operation and were higher than that of group A significantly(P < 0.05).The levels in group C were lower than that in group B significantly,especially in the 16th points(P < 0.05).The histopathological changes showed that the myocardial tissues were more severe in group B than that in other groups;Myocardial tissues in group C were eased significantly compared to that of group B.Conclusion: CVF can reduce the levels of both complement C3 in serum and MPO,lessen the myocardial injury in septic rats significantly,which showed the protective effects on myocardial tissues of septic rats.更多还原