To provide genetic markers for the study of ecological adaptability to responsive droughtand salinity-stress for Medicago ruthenica Trautv.,we selected eight pairs of good polymorphism and high stability primers from 70 pairs of drought-and salinity-responsive EST-SSR markers published for chickpea.DNA sequences were amplified with eight pairs of SSR primers by SSR-PCR technique.One hundred and eleven alleles were detected,on average,13.88 alleles were detected per site.The mean values of observed heterozyg...