【摘要】 目的:探讨细胞色素2J3(CYP2J3)基因转染减轻动脉球囊损伤后血管狭窄程度的初步机制。方法:36只实验大鼠,其中18只用于建立胸主动脉球囊损伤模型,余18只进行假手术。前者分为三组(n均=6),分别从尾静脉注入生理盐水,为动脉损伤模型组(I组)、CYP2J3(2J3I组)、pcDNA3.1(3.1I组);后者亦分为三组(n均=6),亦从尾静脉注入与上相同试剂,分别为假手术组(Sh组)、2J3Sh组和3.1Sh组。术后28天取各组大鼠胸主动脉,观察动脉环相对管腔面积(RLA)变化,检测细胞外信号调节激酶(ERK1/2)、内皮型一氧化氮合成酶(eNOS)蛋白表达。结果:动脉损伤各组大鼠RLA均小于相应Sh组(P<0.05或P<0.01);2J3I组RLA大于I组及3.1I组(P<0.01)。动脉损伤各组较相应Sh组磷酸化ERK1/2蛋白表达升高(P<0.05或P<0.01),2J3I组ERK1/2蛋白表达高于I组和3.1I组(P均<0.01)。动脉损伤各组eNOS蛋白表达低于相应Sh组(P<0.01);2J3I组eNOS蛋白表达高于I组和3.1I组(P均<0.01)。结论:CYP2J3基因转染可减轻动脉损伤后狭窄,其作用与其增加ERK1/2及eNOS蛋白表达有关。更多还原

【Abstract】 Objective:To investigate the initial mechanism of CYP2J3gene transfection lessen vascular stenosis after aortic sac injury.Method:Rats were divided into six groups randomly:I,Sh,2J3I,2J3Sh,3.1Iand 3.1Sh groups.28days after artery sac injury,the RLA,ERK1/2and eNOS protein levels in thoracic aorta were detected.Results:The data showed that the RLA of 2J3Igroup was higher than I group and 3.1I group(P<0.01).About ERK1/2protein content,2J3Igroup was higher than 3.1Igroup(P<0.01);however,the eNOS protein content of 2J3Igroup was higher than I group and 3.1Igroup(P<0.01).Conclusion:Up-regulating CYP2J3/EETs system can decrease the degree of artery stenosis after injury,which is relevant with increasing ERK1/2and eNOS protein levels.更多还原