【摘要】 目的研究铜缺乏对大鼠肝脏铁调节蛋白2(IRP2)、转铁蛋白受体(TfR)及铁蛋白(Fn)mRNA的影响。方法 40只雄性SD大鼠按体重随机分为4组,每组10只,对照组(Ⅰ组),铜完全缺乏组(Ⅱ组),铜边缘缺乏组(Ⅲ组),铁及铜边缘缺乏组(IV组)。喂养8周后处死,取大鼠组织和血清,进行相关指标和基因表达检测。结果与对照组比较,铜缺乏使大鼠血清铁和血清铁蛋白含量显著降低(P<0.05),血清转铁蛋白受体水平明显升高,铜缺乏时大鼠肝脏IRP2、TFR mRNA表达水平明显升高,Fn mRNA明显降低(P<0.05)。结论铜缺乏通过影响铁吸收、储存、转运而影响大鼠体内铁的营养状况。铜缺乏影响大鼠IRP2 mRNA表达与IRE-IRP结合活性,在转录后水平改变TfR和Fn mRNA的表达,影响机体的铁稳态。更多还原

【Abstract】 Objective To study the effects of copper deficiency on iron metabolism,the expression of IRP mRNA and Fn mRNA and transferrin receptor mRNA in rats.Methods Forty clean male SD rats were randomly divided into four groups according to body weight and there were 10 rats in each group.The groups are normal iron and copper control group(groupⅠ),copper deficiency group(groupⅡ),normal iron and copper slightly deficient group(group Ⅲ),both iron and copper slightly deficient group(group Ⅳ).After 8 weeks,all the rats were killed by sodium pentobarbital anesthesia and all samples were collected and detected for gene expression.Results Compared with the controls,the contents of serum iron and serum ferritin in completely copper deficiency group decreased(P<0.05),but the level of serum transferrin receptor increased significantly under the complete copper deficiency(P<0.05).Compared with that of the control group,the level of IRP2 mRNA in complete copper deficiency group was significantly increased(P<0.05),and the level of transferrin receptor mRNA was also increased compared with that of the control group,compared with the control group the level of ferrintin RNA in complete copper deficiency group was significantly decreased(P<0.05).Conclusion The nutritional status of the iron may be affected by copper deficiency through influencing the absorption,the results indicate that copper deficiency influences iron homeostasis in cells through affecting the expression of IRP2 and the activity of IRP-RNA combination which change the expressions of ferritin and transferrin mRNA.更多还原