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金黄色葡萄球菌ClfA蛋白Th表位鉴定及其免疫原性分析(英文)

Identification of Th cell epitopes on ClfA adhesin of Staphylococcus aureus and characterization of their role in immunity

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【Author】 Yang Li1 ,Zhijie Li3 ,Yagang Li4,Junfeng Ye2 1Key Laboratory of Zoonosis,Ministry of Education,Institute of Zoonosis,Jilin University,Changchun 130062,China 2First Hospital of Jilin University,Changchun 130021,China 3State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China 4Fourth Hospital of Jilin University,Changchun 130062,China

【机构】吉林大学人兽共患病研究所人兽共患病研究教育部重点实验室；中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室；吉林大学第四医院；吉林大学第一医院；

【摘要】【目的】ClfA蛋白是金黄色葡萄球菌粘附于宿主细胞的最主要粘附因子之一,筛选鉴定ClfA蛋白的Th表位,研制基于表位的联合疫苗将成为该菌疫苗新的发展方向。【方法】本研究采用生物信息学软件预测ClfA可能的H-2d限制性Th表位,并以CD4+T淋巴细胞增殖实验及流式细胞分析进行筛选和鉴定。【结果】获得BALB/c小鼠H-2d限制性Th1表位(C335)和Th2表位(C214,C286和C436)。【结论】筛选出优势性Th细胞表位,并对表位的免疫学特性进行实验免疫研究,为最终研制保护作用强、安全性高的新型疫苗奠定基础。更多还原

【Abstract】 [Objective] The ClfA adhesin of Staphylococcus aureus is an excellent vaccine candidate antigen.CD4+ T cells play central roles during immune responses,but their functional contributions to Staphylococcus aureus in fection have yet to be evaluated.[Methods] By using the SYFPEITHI prediction algorithm,we identified and characterized four Th epitopes within the ClfA adhesin.[Results] Peptide C335 was I-Ed restricted Th1 type epitopes;peptides C214,C286,and C436 were I-Ad restricted Th2 type epitope.[Conclusion] The identification of these epitopes is important to evaluate and optimize the vaccine-primed protection against Staphylococcus aureus infection.更多还原

【关键词】金黄色葡萄球菌； ClfA； CD4+T cell；表位；
【Key words】 Staphylococcus aureus； ClfA； CD4+T cell； Epitope；

【基金】 Supported by National Natural Science Foundation of China(81100326);the China Postdoctoral Science Foundation(2012M520684);the Basic Research Project of Jilin University(450060445322);the Jilin University Personnel Development Fund(4305050102Q5);the Foundation of Jilin Province Technological Committee(20090737);the Jilin Province Postdoctoral Science Foundation(801130020428)~~

【文献出处】微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,2013年09期

【分类号】R392
【被引频次】2
【下载频次】131

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