【摘要】 为分析2009~2011年上海地区手足口病流行病学特征和病原构成,从国家疾病监测信息报告管理系统获取上海市2009~2011年手足口病流行病学资料;采用实时荧光反转录聚合酶链反应(RT-PCR)对来自上海18个区(县)的6 676例手足口病病例标本进行肠道病毒核酸检测,对其中257份标本进行病毒分离;对27份人肠道病毒71型(HEV71)毒株进行VP1基因序列全长测定和分析。结果显示,2009~2011年上海市18个区(县)均有手足口病病例报道,地区分布无显著差异;≤5岁的婴幼儿为疾病高发年龄段;4~11月为发病高峰期。HEV71和柯萨奇病毒A组16型(CA16)为主要病原,不同地区病原构成有所不同。实时荧光RT-PCR对6 676例病例标本进行核酸检测,其中肠道病毒通用核酸检测阳性率为69.61%,HEV71和CA16阳性率分别为38.83%和21.26%。对257份HEV71核酸阳性标本进行病毒分离,获得毒株57株,分离阳性率为22.18%。对其中27株进行VP1基因全长测序,均属C4a基因亚型。更多还原

【Abstract】 The present paper aims to investigate the epidemiological and etiological characteristics of hand, foot and mouth disease(HFMD)in Shanghai from 2009to 2011.The data from the National Notifiable Disease Surveillance System were analyzed.A total of 6 676clinical samples were collected and real-time reverse transcriptase-polymerase chain reaction(RT-PCR)was performed to determine the pathogens.The results showed that 18districts/counties had reported HFMD cases;The children ≤5years old were the most susceptible population;The peak of HFMD epidemic was from April to November;Human enterovirus 71(HEV71)and coxsackievirus A16(CA16)were the major pathogens for this epidemic,but the constituent ratio of HEV71and CA16 was different in different months and regions.Out of the 6 676 patients,4 647cases were positive for enterovirus by real-time RT-PCR,The positive rate was 69.61%. Among them 1 419were CA16(the overall positive rate of 21.26%)and 2 592were HEV71(the overall positive rate of 38.83%).257HEV71samples were cultured with human rhabdomyosarcoma(RD)cells, and 57HEV71strains were obtained and 27of these were subjected to sequence analysis.The complete sequences of VP1-encoding region in several identified HEV71strains were analyzed.BLAST sequence analysis showed that all the 27isolated HEV71strains from 2009to 2011were classified into C4a.更多还原