【摘要】 目的研究过氧化氢诱导HPF细胞早衰与细胞复制性衰老的生物学性状异同。方法 200μmol/L H2O2染毒HPF细胞4次制备过氧化氢诱导细胞早衰模型;常规培养细胞,固定传代,直至细胞停止增殖以制备细胞复制性衰老模型。动态观测两种模型细胞的生长形态、生长特性、细胞衰老特异性β-半乳糖苷酶染色及细胞周期的变化情况。结果细胞复制衰老模型在群体倍增次数(population doubling level,PDL)至54代时细胞停止增殖,200μmol/L H2O2诱导细胞在PDL34进入衰老;正常细胞的细胞周期PI指数与群体倍增次数负相关;与PDL50组细胞相比,200μmol/LH2O2染毒组细胞的群体倍增时间和β-半乳糖苷酶染色差异均无统计学意义(P>0.05),PI指数差异有统计学意义(P<0.05)。结论 200μmol/L H2O2可诱导细胞早衰,其生物学性状与细胞复制性衰老模型不完全一致。更多还原

【Abstract】 Objective To study biological characteristics of replicative cell senescence and oxidative-stress induced premature senescence.Methods HPF cells were treated with 200 μmol/L H2O2 four times to induce cell senescence.HPF cells were continuously cultured and subcultured in vitro until losing their reproductive capacity.The cell morphology,growth curve,population doubling time,senescence-associated β-galactosidase and cell cycle were observed.Results The normal HPF cells and 200 μmol/L H2O2 treated cells stopped replication on PDL54 and PDL34 respectively.In normal cells,PI was negatively correlated with population doubling level.There were no significant differences in cell population doubling time and senescence-associated β-galactosidase expression between PDL50 group and 200 μmol/L H2O2 group.PI of 200 μmol/L H2O2 group was significantly different from PDL50 group.Conclusion The concentration of 200 μmol/L H2O2 could induce premature senescence.Although the biological characteristics of premature senescence cells is similar to replicative senescence cells,there are different mechanism.更多还原