【摘要】 目的探讨胃泌素及其与N-甲基-N’-硝基-N-亚硝基胍(MNNG)合用时对大鼠胃黏膜的影响。方法 Wistar大鼠共24只,分为4组:空白对照组(A组,6只):自由饮用纯净水;胃泌素组(G组,6只):连续每天皮下注射胃泌素300μg/kg共90d;MNNG组(M组,6只):饮用含MNNG100μg/ml的自来水;胃泌素与MNNG合用组(M+G组,6只):饮用含MNNG100μg/ml的自来水,同时连续注射胃泌素每天300μg/kg共90d。第90天取材,比较各组胃的组织学变化,并采用免疫组化的方法检测bcl-2(即B细胞淋巴瘤/白血病-2基因)和ki-67(增殖细胞核抗原)抗体在不同胃组织中的表达。结果 4个组动物胃黏膜层厚度差异有统计学意义(P<0.05),G组(964.70±170.5)μm及M+G组(817.68±109.5)μm与A组(785.43±98.3)μm和M组(713.50±92.3)μm相比,胃黏膜层增加。G组、M组及M+G组ki-67及bcl-2的表达量均增加,且与A组比较,差异均有统计学意义(P<0.05)。结论胃泌素能促进胃黏膜生长增厚且增加ki-67及bcl-2的表达量,提示单独高胃泌素血症有可能通过促进细胞增殖及抑制细胞凋亡而最终导致肿瘤的形成。更多还原

【Abstract】 Objective To investigate the effect of gastrin on rats model of gastric mucosa with N-methyl-N’-nitro-Nnitrosoguanidine ( MNNG) . Methods 24 Wistar rats were divided into 4 groups: blank control group ( group A,n = 6) was given water alone,gastrin group ( group G,n = 6) was given pentagastrin 300 μg / kg for 90 days,MNNG group ( group M,n =6) was given MNNG water alone,gastrin and MNNG group ( group M + G,n = 6) were given MNNG water and pentagastrin 300 μg / kg for 90 days. After 90 days,rats were euthanized. Gastric mucosa was evaluated histologically. The expression of bcl-2 and ki-67 were examined with immunohistochemical staining technique. Results There were difference in gastric mucosal layer thickness among 4 groups ( P < 0. 05) ,compared with group A and group M,mucosal thickness in group G and group M + G were increased. Compared with group A,expression of bcl-2 and ki67 in group G,group M and group M + G were higher,the raise was statistically significant ( P < 0. 05) . Conclusion Gastrin can promote the growth of the gastric mucosa and increase the expression of ki-67 and bcl-2,it suggests that gastrin alone may contribute to malignant change through promoting cell proliferation and inhibiting apoptosis.更多还原