【摘要】 目的:探讨有氧运动和G-CSF干预对心梗(MI)大鼠心肌细胞再生和心功能提升的影响及其可能机制。方法:雄性SD大鼠,体重180～220g,建立MI模型。术后1周将存活大鼠随机分为假手术组(Sham组)、心梗组(MI组)、心梗+运动组(ME组)、心梗+动员剂组(MG组)和心梗+动员剂+运动组(MGE组),每组15只。ME和MGE组进行8周跑台有氧运动。8周后测定心功能,进行组织学染色,检测细胞增殖和干细胞趋化相关因子的表达。结果:与Sham组比较,MI组心肌出现替代性纤维化,心功能显著下降,心肌中PCNA、Ki-67和VLA-4表达增多(P<0.01),GATA 4mRNA和蛋白表达下降(P<0.01,P<0.05);与MI组比较,ME、MG和MGE组梗死区心肌纤维化程度减轻,心功能显著提升,梗死面积显著缩小(P<0.05),心肌中PCNA、Ki-67、c-kit、CD29、CXCR4和VLA-4蛋白的表达显著增加;且二者联合干预使LVEDP下降和梗死面积缩小更为显著(P<0.05),细胞增殖和干细胞趋化相关因子表达增加优于单一因素。结论:有氧运动和/或G-CSF干预均可缩小梗死面积,有效提升大鼠心功能;发现有氧运动和G-CSF干预可有效促进MI后心肌细胞增殖及成体干细胞动员、归巢和分化,且二者联合干预效果更为显著。更多还原

【Abstract】 Objectives:To discuss the influence and possible mechanism of aerobic exercise(AE) and granulocyte colony-stimulating factor(G-CSF) treatment on myocardial regeneration and improvement of cardiac function in rats with myocardial infarction(MI).Methods:Adult male sprague-dawley rats,weight about 180~220g were randomly divided into:Sham-operated group(Sham),Sedentary MI group(MI),MI with AE group(ME),MI with G-CSF treatment group(MG) and MI with AE plus G-CSF treatment group(MGE).The MI model in rat was established by ligation of the left anterior descending(LAD) coronary artery,and rats in ME and MGE were subjected to 8-week treadmill training.At the end of the 8-week training,hemodynamic measurement was performed to evaluate cardiac function;hearts were picked for TTC dyeing and histological section;the expression levels of factors related to cell proliferation and stem cell homing were detected by immunohistochemical measurement,western blot or RT-PCR.Results:Compared to Sham,the MI rats were found to have impaired cardiac function and increased cardiac fibrosis,the expression levels of PCNA,Ki-67 and VLA-4 increased(P<0.01),and mRNA and protein levels of GATA4 decreased(P<0.01,P<0.05);compared to MI rats,AE or G-CSF treatment ameliorated cardiac remodeling,improved cardiac function,decreased infarct size(P<0.05),and increased the expression of PCNA,Ki-67,c-kit,CD29,CXCR4,VLA-4 in ME and MG rats;the combined treatment had a better effect than pure intervention factors.Conclusion:AE or G-CSF treatment significantly improved myocardial remodeling and cardiac function in MI rats,and increased myocardial cells regeneration and activation of the stem cell movement.The better effects were appeared with the combined treatment.更多还原