【摘要】 以实验室保藏的一株具有溶解血栓能力的芽孢杆菌(LJQ-NK)为出发菌株,通过形态学分析和16SrRNA比对,对菌株进行鉴定,更进一步利用聚合酶链式反应(PCR)克隆纳豆激酶DNA片段⑤16SrRNA比对。结果表明LJQ-NK与已报道的纳豆激酶16SrRNA序列有99.9%以上的同源性,初步认定为一株纳豆芽孢杆菌。克隆获得1 120 bp的DNA片段,应用DNAMAN软件分析,与已报道的纳豆激酶编码基因(GI29164926)存在两个碱基的差异,编码纳豆激酶开放阅读框区域序列完全相同。更多还原

【Abstract】 LJQ-NK,a strain with ability of fibrinolysis was identified by molecular biology method.Sequence analysis shows that the homology of 16S rRNA between LJQ-NK and Bacillus subtilis natto was exceeded 99.9%.The putative nattokinase cDNA was cloned from LJQ-NK using RT-PCR and a 1 126 bp cDNA clone was obtained.Result of BLAST-X sequence homology search show that two bases of putative nattokinase cDNA clone were differed from nattokinase and the ORF sequence of nattokinase between LJQ-NK and Bacillus subtilis natto were identical.更多还原