【摘要】 以海滨锦葵籽粒脱脂粉为原料,采用超声波法、DEAE-纤维素柱层析、浓度梯度洗脱模式(洗脱液盐浓度分别为0.1、0.25、0.4 mol/L)分离纯化得到三个蛋白组分;再对0.1 mol/L盐洗脱蛋白进行超滤分离。采用SDS-PAGE测定纯化蛋白分子量的分布,并利用MTT法测定蛋白的体外免疫和抗肿瘤活性。结果表明,总水溶性蛋白得率为1.12%;0.25 mol/L盐洗脱冻干粉的蛋白质量分数最高为58.8%;含量较高的蛋白质分子量分别为23、32和60KD;0.1 mol/L盐洗脱蛋白表现出较好的促进脾淋巴细胞增殖和抑制HepS细胞增殖的作用。随着时间延长,小于30KD的超滤蛋白组分对HepS细胞生长表现出显著或极显著抑制效果。更多还原

【Abstract】 Degrease powder of Kosteletzkya virginica seeds were used as raw materials in this research. Three components of protein were purifiedby ultrasound,DEAE-cellulose column chromatography with gradient concentration desorptionmode( the concentrationsof desorption saltwere 0. 1,0. 25 and 0. 4 mol / L,respectively). 0. 1 mol / L salt desorption components were further separated using ultrafiltration. Molecular weight distribution of three protein components were determined by SDS-PAGE. In vitro immunization and antitumor activities of proteins were tested by MTT assay. The results showed that the total soluble protein extraction rate was 1. 12%; the highest protein mass percent was 58. 8% from 0. 25 mol / L salt desorption concentration; with higher levels of protein molecular weight 23,32 and 60KD. 0. 1 mol / L salt desorption protein( SDP) showed significant effects on promoting proliferation of splenic lymphocyte and inhibiting proliferation of mouse hepatoma cell( HepS). Components of smaller than 30KD showed significant and very significant inhibitory effects on proliferation of HepS cells with the increasing of time.更多还原