【摘要】 采用HPLC测定脱落酸(abscisic acid,ABA)处理1、3、6、9 d后丹参毛状根中丹参酮I、隐丹参酮、二氢丹参酮I、丹参酮IIA含量的方法,研究不同ABA处理时间对丹参酮类成分积累的影响。结果表明:①随着ABA处理时间的增加,丹参毛状根生长受到抑制逐渐减弱,并在处理6 d左右变的不显著;②毛状根中四种丹参酮的产量和含量均在ABA处理6 d左右趋于稳定。其中丹参酮I、隐丹参酮、二氢丹参酮I和丹参酮II的产量分别提高为空白对照组的4.65、7.80、18.33和2.11倍;③三种丹参酮类合成抑制剂均对丹参毛状根的生长、丹参酮类的合成有抑制作用。更多还原

【Abstract】 The objective of this study was to investigate the effects of abscisic acid(ABA) treatment times on the accumulation of active constituents of Salvia miltiorrhiza hairy root.The contents of tanshinones(including tanshinone I,cryptotanshinone,dihydrotanshinon I and II A) in S.miltiorrhiza hairy root was quantified after 0,1,3,6,9 days’ treatment of ABA by HPLC.The results showed that:1) the growth of S.miltiorrhiza hairy roots was inhibited with the ABA treatment.The inhibition effect was decreased with the increasing of treatment time and became stable at day 6;2) The yields and the contents of four tanshinone components of S.miltiorrhiza hairy roots were also stable at day 6.the yields of tanshinone I,cryptotanshinone,dihydrotanshinon I and II A were 4.65,7.80,18.33 and 2.11 times of the control group after dealing with ABA;3)The root growth and the tanshinone production were inhibited efficiently by FOS and MEV and TUN.更多还原