【摘要】 本文研究红毛五加多糖不同组分(AHP-I、AHP-II、AHP-III)对小鼠腹腔巨噬细胞免疫调节功能的影响,为进一步阐明红毛五加多糖对小鼠免疫调节作用机制奠定基础。采用不同浓度的3种多糖组分作用于小鼠腹腔巨噬细胞,测定其对巨噬细胞吞噬中性红、释放NO能力、分泌IL-6、TNF-α、IL-1β水平的影响。最后结果是红毛五加多糖的3种不同组分对小鼠免疫细胞有不同的刺激能力。其中,AHP-II可极其显著地增强吞噬细胞的吞噬功能,促进其合成NO,促进巨噬细胞细胞因子的分泌。因此红毛五加多糖能激活小鼠腹腔巨噬细胞,其中,AHP-II是最重要的作用组分。更多还原

【Abstract】 The objective of study was to investigate the effects of different components of Acanthopanax Giraldii Harms polysaccarides(AHP-I,AHP-II,AHP-III)on the immunoregulation function of the peritoneal macrophage of mouse.The engulfment of neutral red and the expression levels of NO,IL-6,TNF-α,IL-1β were determined for all of these three fractions.The Results showed that the polysaccharides from Acanthopanax Giraldii Harm have different stimulating activities on mouse’s peritoneal macrophage cells.The AHP-II enhanced the phagocytic activity significantly,and promoted the release of NO and other cytokines.All of the three polysaccharides activated the mouse’s peritoneal macrophage cells,and the effect of AHP-II was the highest.更多还原