【摘要】 本文研究了壁虎乙醇提取物(GEE)诱导Hep2细胞凋亡的作用。采用噻唑蓝比色法(MTT)检测了GEE对Hep2细胞增殖的抑制作用,发现GEE可呈剂量-时间依赖性的抑制Hep2细胞的增殖,GEE作用24、48、72 h后其IC50值分别为336.858、237.949、188.991μg/mL。Hoechst 33258荧光染色后在显微镜下可观察到Hep2细胞发生了典型凋亡的形态学变化;利用Western blot法证实在Hep2细胞中GEE可诱导caspase-3和抑制VEGF蛋白的表达。这些结果表明GEE可能是通过上调Caspase-3和下调VEGF蛋白的表达诱导Hep2细胞的凋亡。更多还原

【Abstract】 The current study explored the apoptosis-inducing activities of Gekko ethanol extract(GEE) on Hep2 cell line.The MTT assay showed that GEE can significantly inhibit the proliferation of Hep2 cells in a time-dose dependent manner.After the treatment of GEE for 24 h,48 h and 72 h,the 50% inhibitory concentration(IC50) was 336.858,237.949 and 188.991 μg/mL,respectively.The typical apoptotic morphological changes of Hep2 cells treated with GEE was observed by Hoechst 33258 staining.Furthermore,the results of western blot assay indicated that GEE can up-regulate the protein expression level of caspase-3 and suppress the expression of VEGF.In conclusion,these findings provided experimental evidences that GEE may have apoptosis-inducing effects on Hep2 cells by increasing protein expression of caspase-3 and down-regulating VEGF.更多还原