【摘要】 从白三叶草(Trifolium repens L.)的叶中分离得一株真菌,鉴定为烟曲霉(Aspergillus fumigatus),采用大米固体培养基发酵,其乙酸乙酯提取物具有抑制白菜种子发芽的能力,并从其中分离得4个生物碱化合物,通过波谱及理化性质鉴定为:pyripyropene A(1)、verruculogen(2)、fumigaclavine C(3)、fumiquinazoline J(4),其中化合物1～3采用钙流模型测定其生物活性,结果显示无活性。更多还原

【Abstract】 A strain of endophytic fungus was isolated from the leaves of Trifolium repens L.,and identified as Aspergillus fumigatus.The fungus was cultured on rice medium.The ethyl acetate extraction portion showed the activity of inhibiting germination of cabbage seeds.In this study,four alkaloids were isolated from the ethyl acetate extraction portion.On the basis of spectroscopic data,their structures were elucidated as pyripyropene A(1),verruculogen(2),fumigaclavine C(3),and fumiquinazoline J(4).In addition,the bioactivity of compounds 1-3 were evaluated by intracellular calcium influx model.However,all the compounds were inactive.更多还原