【摘要】 目的研究Musashi-2、CD133在结肠腺癌组织和正常结肠黏膜组织中的表达及其与结肠腺癌发生发展的关系。方法采用免疫组织化学法检测并比较40例结肠癌组织(结肠腺癌组)及15例正常结肠黏膜组织(对照组)中Musashi-2和CD133蛋白的表达,分析结肠腺癌组Musashi-2、CD133蛋白的表达与临床病理参数的关系。结果结肠腺癌组Musashi-2、CD133蛋白的阳性表达率分别是60%、27.5%,对照组分别是26.7%和0,结肠腺癌组均高于对照组(χ2分别为4.850、5.156,均P<0.05)。组织分化程度越低、TNM分期越高,Musahi-2蛋白的阳性表达率越高;组织分化程度越低、出现淋巴结转移,CD133蛋白的阳性表达率越高(均P<0.05)。结论Musashi-2和CD133可能跟结肠腺癌的发生发展有关,可将其做为结肠腺癌干细胞标志物进一步研究。更多还原

【Abstract】 Objective To investigate the expression of Musashi-2 and CD133 in colonic adenocarcinoma,and their correlation with the occurrence and development of colonic adenocarcinoma thereof.MethodsThe expressions of Musashi- 2 and CD133 protein were detected by immunohistochemical method in 40 colonic adenocarcinoma samples and 15 normal colonic structure samples. The different histological types, TNM staging, lymph node metastasis, serous infiltration, distant metastasis and expressions of Musashi-2 and CD133 proteins in colonic adenocarcinoma tissues were analyzed.Results The positive expression rates of Musashi-2 and CD133 protein were 60% and 27.5% in 40 colonic adenocarcinoma samples and 26.7% and 0 in 15 normal colonic samples, which showed the significantly higher expression rates in colonic adenocarci noma group than those of control group(χ2=4.850 and 5.156,P < 0.05). There were significant differences in expressions of Musashi-2 proteins between different histological stages and TNM staging(P < 0.05). The positive expression rate of Musa hi-2 protein increased as the degree of differentiation decreased and TNM stage increased. There were significant differenc es in expressions of CD133 proteins between different histological stages and lymph node metastasis(P < 0.05). The positive expression rate of CD133 protein increased as the degree of differentiation decreased and lymph node metastasis occurred. ConclusionThe expression of Musashi-2 and CD133 may be related with the initiation and development of colonic can cer, which can be used as the stem cell markers of colonic adenocarcinoma for the further study.更多还原