【摘要】 目的探讨乙醇对成骨细胞凋亡的影响。方法体外培养新生大鼠成骨细胞作为细胞模型,对照组采用含10%FBS的1640培养液培养;干预组在对照组正常培养1d后,加入100mmol/L乙醇。取第3代成骨细胞按实验分组,于乙醇作用后1、3、5、7d进行形态学观察及成骨细胞增殖的检测,于乙醇作用后3、7、10、14d进行成骨细胞分化指标检测,于乙醇作用后1d采用流式细胞仪对成骨细胞凋亡进行检测。结果2组第1天细胞增殖差异无统计学意义(P>0.05),干预组第3、5、7天细胞增殖率低于对照组,差异有统计学意义(P<0.01)。培养后3、7、10、14d,对照组细胞碱性磷酸酶(ALP)、骨钙素(BGP)逐渐升高,干预组细胞ALP、BGP逐渐降低(P<0.05);同期比较,干预组细胞ALP、BGP含量低于对照组(P<0.01)。对照组成骨细胞1d自发性凋亡率低于干预组[(1.89±0.63)%vs(2.94±0.91)%],差异有统计学意义(P<0.01)。结论一定浓度的乙醇可以诱导体外培养成骨细胞发生凋亡,造成其增殖与分化能力逐渐减弱。更多还原

【Abstract】 Objective To study the influence of alcohol on osteoblast apoptosis in vitro. Methods The mouse calvarial osteoblasts were cultured in vitro to three generations and divided into two groups: the control group and intervention group. Cells of control group were cultured in 1640 medium containing 10% FBS, and cells of intervention group were added 100 mmol/L alcohol after 1 day normal culture. After 1 , 3 , 5 and 7d culture, cells were harvested to measure MTT value. After 3 , 7 , 10 and 14 d culture, cells were harvested to measure alkaline phosphatase (ALP) and osteocalcin (BGP) activity. After 1 d culture, the osteoblast apoptosis was assessed by flow cytometry. Results There was no significant difference in the value of MTT between two groups (P > 0.05). The cellular proliferation rate was significantly lower in intervention group after 3, 5 and 7 d culture (P < 0.01). The values of ALP and BGP significantly increased in control group and decreased in intervention group after 3, 7, 10 and 14 d culture (P < 0.05 or P < 0.01). The values of ALP and BGP were significantly lower in interven tion group than those in control group after 3, 7, 10 and 14 d culture (P < 0.01). The apoptotic rate was significantly higher in intervention group than that in control group after 1 d culture (P < 0.01). Conclusion Alcohol had a marked effect on osteoblast apoptosis by weakening the proliferation and differentiation.更多还原