【摘要】 目的:分离培养并鉴定人脂肪源性间充质干细胞(hAD-MSC)。方法:采用贴壁法从人脂肪组织分离获得hAD-MSC,并从表面分子表达和定向分化两个方面进行鉴定。结果:成功从脂肪组织中分离获得间充质干细胞,该群细胞贴壁生长,形态类似成纤维细胞样;具有较强的体外增殖能力和自我更新能力;一致性好,纯度高,99%的细胞阳性表达CD105、CD90、CD13、CD44分子;CD117、CD34、CD45和HLA-DR阴性表达,阳性率低于2%;可向脂肪和成骨细胞定向分化。结论:分离所得细胞符合间充质干细胞基本特点,可确定为hAD-MSC。更多还原

【Abstract】 Objective: To isolate and expand human adipose derived mesenchymal stem cells(hAD-MSC) from adult adipose tissue,and define the characteristics of these cells.Methods: hAD-MSC were isolated from adipose tissue by collagenase digestion and adherence to plastic,and the surface marker and potential multipotent differentiation ability of these cells were identified.Results: Fibroblast-like cells were obtained from adipose tissue.These cells could be expanded quickly in vitro,and showed self-renewal ability.CD105,CD90,CD13 and CD44 were expressed in 99% of isolated cells while CD117,CD34,CD45 and HLA-DR expression was negative.They could be induced to differentiate osteoblasts and adipocytes in special differentiation medium.Conclusion:These cells from human adipose are hADMSC.更多还原