【摘要】 目的探讨周氏克金岩方促进舌苔形成相关细胞凋亡的作用机制。方法体外培养SAS和TCA-8113舌鳞癌细胞,撤除血清模拟舌苔形成相关细胞凋亡环境,周氏克金岩方正丁醇部位作用舌鳞癌细胞,MTT法检测细胞增殖活性,流式细胞术检测细胞周期分布和凋亡,ELISA检测细胞培养上清PGE2含量。结果与对照组相比,周氏克金岩方丁醇部位能够降低舌鳞癌细胞的增殖活性,促进SAS和TCA-8113细胞凋亡率升高,抑制细胞分泌PGE2,且具有剂量和时间依赖性。结论周氏克金岩方正丁醇部位促进舌苔形成相关细胞凋亡与抑制PGE2合成相关。更多还原

【Abstract】 ObjectiveTo investigate the mechanism of Zhou’s prescription on inducing apoptosis related to the formation of tongue coating. MethodsThe two kinds of human tongue squamous cell carcinoma were cultured in RPMI-1640 with serum to simulate the damnification and rehabilitate of the lingual dorsum epidermis in vitro.The effective part which was extracted from Zhou’s prescription by chemical methods,role in the cells.After the two kinds of human squamous cell carcinoma were incubated in RPMI-1640 with Zhou’ s prescription,MTT assay evaluated the activities of cell proliferation,and flow cytometric analysis was used to analyze the cell cycle distribution and apoptosis.Detected the level of the PGE2 in the cell culture supernatant with enzyme-linked immunosorbent assay(ELISA). ResultsIn comparison with the control,MTT assay,of nhich the result showed the Zhou’s prescription could induce inhibitory effects on the growth in SAS and TCA-8113 cells.The flow cytometric analysis showed that the Zhou’s prescriptionc could induce the apoptosis of human squamous cell carcinoma.Besides the result of The ELISA assay result showed that with the increasing of the concentration of the component,the Zhou’s prescription could decrease the PGE2 level in the cell culture supernatant. ConclusionThe results suggested that the inhibitory effects of Zhou’s prescription on apoptosis which related to the formation of tongue coating resulted from the inhibition of PGE2 releasing.更多还原