【摘要】 目的探讨白英生物碱(STA)诱导人肺癌A549细胞凋亡及其对Fas途径相关基因表达的影响。方法采用STA处理体外培养的A549细胞,用MTT法检测细胞增殖抑制率,Annexin V-FITC/PI双染后用流式细胞仪检测细胞凋亡,Western blot检测Fas、FasL、Cleaved caspase-8和Cleaved caspase-3蛋白表达。结果 STA能抑制A549细胞增殖,STA各组细胞抑制率显著升高且呈剂量依赖性,与正常对照组比较差异有统计学意义(P<0.01);STA各组细胞凋亡率明显升高,与正常对照组比较差异有统计学意义(P<0.01);Caspase-8/Caspase-3抑制剂能抑制STA诱导细胞凋亡,与同剂量STA组比较差异有统计学意义(P<0.05或P<0.01);STA各组细胞Fas、FasL、Cleaved caspase-8和Cleaved caspase-3表达显著上升,与正常对照组比较差异有统计学意义(P<0.05或P<0.01)。结论 Fas介导的死亡受体途径参与STA诱导肺癌A549细胞凋亡。更多还原

【Abstract】 Objective To investigate the effects of solanum lyratum thunberg alkaloid(STA)on induction of apoptosis and the expression of Fas-pathway related genes in A549 cells. Methods A549 cells were treated with STA in vitro.The proliferation inhibitory rate was evaluated by MTT assay.Induction of cell apoptosis rate was determined by flow cytometry(FCM)method after Annexin V-FITC/PI double staining.The expression of Fas,FasL,Cleaved caspase-8 and Cleaved caspase-3 were detected by western blot. Results STA could inhibit the proliferation of A549 cells.The inhibitory rate was increased obviously with dose dependency in STA groups,compared with the control group,the difference was statistically significant(P<0.01).The apoptotic rate was increased significantly in STA groups,compared with the control group,the difference was statistically significant(P<0.01).Caspase-8/Caspase-3 inhibitor could suppress the apoptosis of A549 cells induced by STA,compared with the same dose of STA group,the difference was statistically significant(P<0.05 or P<0.01).The expression of Fas,FasL,Cleaved caspase-8 and Cleaved caspase-3 proteins were increased markedly in STA groups,compared with the control group,the difference was statistically significant(P<0.05 or P<0.01). Conclusion Fas-mediated death receptor pathway is involved in STA inducing lung adenoearcinoma A549 cells apoptosis.更多还原