【摘要】 目的:观察荔枝核总黄酮(total flavone of litchi chinensis sonn,TFL)对肝纤维化大鼠肝组织转化生长因子-β1(TGF-β1)和骨桥蛋白(osteopontin,OPN)表达的影响。方法:雄性SD大鼠80只,随机分为假手术组,模型组,TFL大、小剂量组。采用胆总管结扎术制备肝纤维化大鼠模型。术后第4周处死大鼠,放免法检测血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)的水平。HE和Masson染色观察肝组织学变化及纤维化程度;免疫组化观察肝组织TGF-β1和OPN的染色部位及强度。结果:TFL大剂量组血清HA、LN、PCⅢ、CⅣ水平以及肝组织TGF-β1、OPN的表达水平均低于模型组和TFL小剂量组,差异具有统计学意义(P<0.05),假手术组和TFL大剂量组间以及模型组和TFL小剂量组间差异无统计学意义(P>0.05)。结论:大剂量TFL可减轻胆汁淤积性肝纤维化大鼠肝损伤及有效改善肝纤维化程度;抑制TGF-β1、OPN高表达,从而减少细胞外基质(ECM)的分泌可能是其抗肝纤维化的机制之一。更多还原

【Abstract】 Objective To investigate the effects of total flavone of litchi chinensis sonn(TFL) on the expressions of transforming growth factor-β1(TGF-β1) and osteopontin(OPN) in rats with hepatic fibrosis. Methods Total 80 males SD rats were randomly divided into 4 groups: sham-operated group(SO group),model group(M group),high-dose TFL group(HD group),low-dose TFL group(LD group). Rat models with hepatic fibrosis were constructed by common bile duct ligation. All rats were sacrificed 4 weeks after the administration,and serum levels of hyaluronic acid(HA),laminin(LN),procolagen type Ⅲ(PC Ⅲ) and Ⅳ collagen(C Ⅳ) were detected by radioimmunoassay. HE and Masson staining were conducted in hepatic tissues to observe the pathological variations; and the expressions of TGF-β1 and OPN were detected by immunohistochemistry. Results Serum levels of HA,LN,PC Ⅲ and C Ⅳ,and the expressions of TGF-β1 and OPN in hepatic tissues in HD group were significantly lower than those in LD group and M group(P < 0.05). Conclusion High-dose of TFL may effectively ameliorate liver injury and hepatic fibrosis through the mechanisms of inhibiting the high expressions of TGF-β1 and OPN and decreasing the secretion of ECM.更多还原