【摘要】 目的:探讨原因不明复发性流产(URM)患者外周血T细胞、NK细胞和调节性T细胞(regulatory T cells,Treg)表型。方法:采用流式细胞术检测URM患者、正常早孕(NEP)和正常非孕妇女(NNP)外周血T细胞(CD3+、CD4+、CD8+T细胞、CD3+HLA-DR+、CD4+HLA-DR+、CD8+HLA-DR+、CD4+/CD8+比值水平)、NK细胞(CD3-CD56+、CD56dimCD16+、CD56brightCD16-、CD56+CD69+)和Treg细胞(CD4+CD25+CD127dim)表型。结果:URM组CD3+HLA-DR+、CD8+HLA-DR+T淋巴细胞均较NEP组明显增加(P<0.05),而CD4+T细胞较NNP组升高(P<0.05)。NEP组CD8+T细胞较NNP组显著增加(P<0.05),三组间CD3+T细胞及CD4+/CD8+比值无明显差异。URM组外周血CD3-CD56+、CD56dimCD16+NK细胞比例明显高于NEP组,而CD56brightCD16-NK细胞比例较NEP组和NNP组均降低(P<0.05)。URM组和NNP组CD4+CD25+CD127dimTreg比例较NEP组均减少(P<0.05)。结论:URM患者外周T淋巴细胞活化、CD56dimCD16+NK细胞增多和Treg细胞减少可能是其致病的免疫基础。更多还原

【Abstract】 Objective To investigate the phenotypes of T lymphocytes,NK cells and regulatory T(Treg) cells in peripheral blood of patients with unexplained recurrent miscarriage patients(URM).Methods The phenotypes of T cells(CD3+,CD4+,CD8+T cells and CD4+/ CD8+ratio,CD3+HLA-DR+,CD4+HLA-DR+,CD8+ HLA-DR+),Treg cells(CD4+CD25+CD127dim),and NK cells(CD3-CD56+,CD56dimCD16+,CD56brightCD16-,CD3CD56+CD69+) in peripheral blood of URM patients,normal early pregnant women(NEP),and normal nonpregnant women(NNP) were determined by flow cytometry.Results In URM group,the percentages of CD3+HLADR+T,CD8+HLA-DR+T cells,CD3-CD56+,and CD56dimCD16+NK cells were significantly higher than those in NEP group(P < 0.05);CD56brightCD16-NK cells were significantly lower than that in NEP and NNP group(P < 0.05);and that of CD4+T cells was significantly higher than that in NNP group(P < 0.05).In NEP group,the percentage of CD8+ T cells was considerably higher than that in NNP group(P < 0.05);and that of CD4+CD25+ CD127dimTreg was significantly higher than those in NNP and URM groups(P < 0.05).There was no significant difference in the percentages of CD3+T cells and the ratio of CD4+/ CD8+among the three groups(P > 0.05).Conclusions The increase of CD56dimCD16+NK cells,the activation of T cells,and the reduction of Treg cells may be the pathogenic immune basis of URM.更多还原