【摘要】 目的:研究下调组织蛋白酶B(cathepsin B,CB)对人食管鳞癌EC9706细胞裸鼠移植瘤的抑制作用并探讨其体内抗肿瘤机制。方法:建立裸鼠荷瘤模型,利用CB siRNA和对照siRNA注射荷瘤裸鼠,监测肿瘤生长变化,采用RT-PCR及Western blot法检测治疗前后瘤体内CB及黏附分子Syndecan-1表达的变化。结果:成功构建了CB siRNA转染荷瘤裸鼠模型。与对照siRNA组和空白对照组相比,CB siRNA组荷瘤裸鼠肿瘤体积下降,组间比较差异具有统计学意义(F=483.992,P<0.05)。转染CB siRNA后,CB蛋白及mRNA表达均显著下调,组间比较差异具有统计学意义(F=733.255及932.681,均P<0.01),Syndecan-1蛋白及mRNA表达则均显著升高,组间比较差异具有统计学意义(F=404.234及1509.951,均P<0.01)。结论:CB siRNA可以有效抑制人食管鳞癌EC9706细胞裸鼠移植瘤的生长,并下调CB的表达和上调Syndecan-1的表达,本研究为食管鳞癌的分子治疗提供了一定的理论依据。更多还原

【Abstract】 Objective To investigate the effect of cathepsin B(CB) reduction on tumor growth of xenografted human esophageal squamous cell carcinoma(ESCC) cell line EC9706 in nude mice and to explore the related molecular mechanism.Methods The xenografted tumor model was established,and the CB siRNA and control siRNA was injected into the xenografted nude mice,respectively.The tumor growth was determined.RT-PCR and Western blot assay were performed to detect CB and Syndecan-1 expressions in tumor tissue.Results After CB siRNA injection,the tumor volume was decreased significantly in the CB siRNA group compared with that in the control siRNA group or the blank control group,respectively(F = 483.992,P < 0.05).The CB mRNA and protein expression was lower in the CB siRNA group than that in the control siRNA group or the blank control group,respectively,and there was significant difference among the three groups(F = 733.255 and 932.681,both P < 0.01).Furthermore,Syndecan-1 mRNA and protein expression was higher in the CB siRNA group than that in the control siRNA group or the blank control group,respectively,and there was significant difference among the three groups(F = 404.234 and 1 509.951,both P < 0.01).Conclusion The CB siRNA could efficiently inhibit the growth of the xenografted human esophageal carcinoma cell line EC9706 in nude mice,followed by the down-regulation of CB and the up-regulation of Syndecan-1,which provides the theoretical basis for the molecular therapy of ESCC.更多还原