【摘要】 目的:探讨miR-375在Ⅱ型子宫内膜癌中的表达及对HEC-1-B细胞增殖、凋亡的影响。方法:miRNA芯片分析Ⅱ型子宫内膜癌miRNA表达谱。利用lipofectamine 2000转染HEC-1-B细胞。实时荧光定量PCR检测转染后miR-375的表达。CCK-8检测转染后细胞增殖能力的改变。流式细胞仪检测细胞转染效率及转染后细胞凋亡情况。结果:miR-375在Ⅱ型子宫内膜癌组织中低表达。转染效率达73.43%。转染后实验组miR-375表达量较NC组和对照组明显增加(P<0.05)。实验组较NC组生长明显受抑(P<0.05)。实验组较NC组、对照组凋亡明显增加(P<0.05)。结论:初步证明miR-375抑制Ⅱ型子宫内膜癌细胞增殖并促进凋亡,可能成为Ⅱ型子宫内膜癌基因治疗的靶点。更多还原

【Abstract】 Objective To explore the expression of miR-375 in type Ⅱ endometrial carcinoma and its effects on the proliferation and apoptosis of HEC-1-B cells.Methods miRNA expression of type Ⅱ endometrial carcinoma was analyzed by miRNA microarray.miR-375 mimics was transfected into HEC-1-B cells by lipofectamine 2000,then the expression of miR-375 was detected by quantitative real-time PCR.The proliferation was detected by CCK-8,and the apoptosis and transfection efficiency were detected by flow cytometry.Results MiR-375 was down-regulated in type Ⅱ endometrial carcinoma,and the transfection efficiency was 73.43%.The expression of miR-375 in experiment group after transfection was increased than those in NC group and control group(P < 0.05).As compared to that in NC group,the inhibitory effect of miR-375 in experiment group was confirmed by CCK-8(P < 0.05).There was significant difference in the apoptosis rate(P < 0.05) between experiment group and NC group,control group.Conclusion MiR-375 might inhibit the proliferation and promote the apoptosis of type Ⅱ endometrial carcinoma cells,which may be the new target of gene therapy for type Ⅱ endometrial carcinoma.更多还原