【摘要】 目的观察吡喹酮(praziquantel,PZQ)对日本血吸虫(Schistosoma japonicum,Sj)感染小鼠发挥早期预防或治疗作用的用药时点。方法健康昆明小鼠46只按设计随机分为PZQ灌服后4 h感染组(A)、感染对照组(D)和感染后2 h用药组(B)、4 h用药组(C)、6 h用药组(E)和8 h用药组(F)。人工感染方法,取阳性钉螺按常规释放尾蚴,经小鼠腹部去毛皮肤作定量感染Sj尾蚴(30±2)条/鼠。PZQ灌服按设计分组实施,以300 mg/kg体重作1次性灌胃。于感染后第42 d用生理盐水经门静脉灌注冲虫,计算每鼠虫体数,观察肝表面虫卵结节。结果感染对照组平均检获虫体(26.3±10.24)条/鼠,各鼠肝表面卵结节密布;感染前4 h用药组和感染后2 h和4 h用药两组均未发现虫体,各鼠肝表面显示正常颜色,无卵结节;感染后6 h和8 h用药两组检获虫体平均每鼠分别为(3.8±4.21)条和(14.3±9.6)条,其鼠肝表面虫卵结节存在不同程度可见。结论对实验小鼠在尾蚴暴露前后4 h时点内口服PZQ可达到有效预防或早期治疗Sj感染的作用。更多还原

【Abstract】 Objective To observe the time point of praziquantel(PZQ) administration for early prevention and treatment of infection with Schistosoma japonicum(Sj) in mice.Methods 46 healthy Kunming mice were randomly divided into six groups as follows: Group A(PZQ was administered 4h before Sj infection),Group D(infected control group),Group B,Group C,Group E and Group F(PZQ was administered 2 h,4 h,6 h and 8 h after Sj infection,respectively).Mice were infected by the artificial method,in which Sj cercariae were released from positive snails,and the mice were quantitatively infected with 30±2 Sj cercariae via abdominal skin.One dose of PZQ of 300 mg/kg.bw was intragastrically administered to mice in various groups at different time points as described above.On the 42th day after infection,Sj adult worms were collected from the mice by portal vein perfusion,and their number was counted for each mouse.The egg nodes on the mouse liver surface were observed and photoed.Results In Group D(infected control group),the average number of Sj adult worms/mouse was 26.3±10.24,and a lot of egg nodes were found on their liver surface.Howevere,in Group A(PZQ was administered 4 h before Sj infection),Group B(PZQ was administered 2 h after Sj infection) and Group C(PZQ was administered 4 h after Sj infection),no parasites were collected and no egg nodes were found on the liver surface.The color of the livers were normal.In the Group E(PZQ was administered 6 h after Sj infection) and Group F(PZQ was administered 8 h after Sj infection),the average numbers of Sj adult worms/mouse were 3.8±4.21 and 14.3±9.6,respectively.Various numbers of egg nodes were found on the liver surface.Conclusions Oral administration of praziquantel within 4 h before and after Sj cercariae exposure in laboratory mice can effectively prevent and treat Sj infection.更多还原