【摘要】 目的了解湖南地区即食食品中食源性致病菌的污染状况和耐药性,为预防和控制食源性疾病提供科学依据。方法从湖南地区的农贸市场、超市和餐饮单位采集即食食品(凉拌菜和熟肉制品),依据GB 4789-2010和GB/T4789-2008方法,对样品进行沙门菌等4种食源性致病菌检测,分离菌株使用微量肉汤稀释法进行药敏试验。结果 991份样品共检出食源性致病菌92株,检出率为9.28%,其中检出金黄色葡萄球菌68株,检出率为6.86%,检出沙门菌和单增李斯特菌各12株,检出率为1.21%,未检出大肠埃希菌O157。药敏结果显示金黄色葡萄球菌和沙门菌均具有一定的耐药性,其中金黄色葡萄球菌对苯唑西林的耐药率高达61.76%,而且多重耐药明显。结论湖南地区即食食品存在一定的食源性致病菌污染,应加大卫生监督力度,防止食源性疾病的暴发流行;同时应控制动物饲料中抗生素的添加使用,加强抗生素的管理和耐药性监测。更多还原

【Abstract】 Objective To investigate the contamination status of food borne pathogenic bacteria in instant food in Hunan and to test their antibiotic resistance,so as to provide evidence for the prevention and control of food borne diseases.Methods Salad and cooked meat were collected in food produce markets,supermarkets and catering units in Hunan province.Four food borne pathogenic bacteria including Salmonella in food were identified in accordance with GB 4789-2010 and GB/T 4789-2008.The bacteria were isolated by broth dilution technique to test their drug sensitivity.Results Totally,92 strains of food borne pathogenic bacteria were identified in 991 food samples,with an incidence of 9.28%.The detection rate of Staphylococcus aureus was 6.86%(68/991).The detection rates of Salmonella and Listeria monocytogenes were both 1.21%(12/991).Escherichia coli O157 was not detected.The drug sensitivity test indicated that Staphylococcus aureus and Salmonella were resistant to some antibiotics.The resistance rate of Staphylococcus aureus against oxacillin was up to 61.76%,and its multi-drug resistance was obvious.Conclusions Some instant food in Hunan is contaminated by food borne pathogenic bacteria.The food hygiene supervision should be strengthened to prevent and control the food borne diseases.The management of use of antibiotics in animal feed should be enhanced and the antibiotic resistance should be closely monitored.更多还原