【摘要】 目的:探讨缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)及P53蛋白在前列腺增生(benign prostatic hyperplasia,BPH)及前列腺癌中的表达情况及两者在前列腺癌发病中的作用及其临床意义。方法:采用免疫组织化学SP法检测40例BPH、40例前列腺癌及10例正常前列腺组织中P53及HIF-1α表达情况。结果:P53在正常前列腺组织中不表达,在BPH、癌性前列腺组织中阳性表达率分别为10%(4/40)、80%(32/40),其在BPH组织中表达率高于正常前列腺组织(P<0.05),前列腺癌组织中表达率高于BPH组织(P<0.05)。HIF-1α在正常前列腺组织中不表达,在BPH、癌性前列腺组织中的阳性表达率分别为40%(16/40)、75%(30/40),在BPH组织中表达率高于正常前列腺组织(P<0.05),前列腺癌组织中表达率高于BPH组织(P<0.05)。P53、HIF-1α与前列腺癌的分期有关(P<0.01)。前列腺癌组织中HIF-1α与P53表达呈正相关(r=0.648)。结论:HIF-1α、P53的异常表达在BPH和癌变的发病中起重要的作用。它们可以作为判断前列腺癌临床分期和预后的重要指标。更多还原

【Abstract】 Objective: To evaluate the expression of the hypoxia-inducible factor-1α( HIF-1α) and P53 in benign-prostatic hyperplasia( BPH) and prostatic carcinoma and the significance on prognosis. Methods: Immunohistochemical method was used to detect HIF-1α and P53 in 40 cases with BPH and 40 cases with prostatic carcinoma and 10 normal prostatic tissue. Resutls: P53 was not expressed in normal prostatic tissue,the positive expression rate in BPH and prostatic carcinoma was 10%( 4 /40),80%( 32 /40) respectively. There was significant difference between 3 tissues( P < 0. 05). HIF-1α was not expressed in normal prostatic tissue, the positive expression rate in BPH and prostatic carcinoma was 40%( 16 /40),75%( 30 /40) respectively. There was significant difference between 3 tissues( P < 0. 05). The coexpression of HIF-1α and P53 correlated significantly with the grade of the tumor( P < 0. 01). They had a close correlation between HIF-1α and P53 in prostatic carcinoma( r = 0. 648). Conclusions: Overexpression of HIF-1α and P53 may play important roles in the pathogenesis of BPH and prostatic carcinoma. They might serve as indicator to the clinical stage and prognosis of prostatic carcinoma.更多还原