【摘要】 目的:建立小鼠酒精性肝损伤模型,探讨有氧运动对小鼠酒精性肝损伤后肝功能的影响以及血液白细胞介素-10(IL-10)、白细胞介素-6(IL-6)和总抗氧化能力(T-AOC)等的变化。方法:40只8周龄小鼠随机分为N组(对照组)、E组(运动组)、AL组(酒精灌胃组)和E+AL组(运动+酒精灌胃组),分别施加有氧运动、酒精灌胃干预。10周后处死取脾脏、肝脏和血清,分别检测IL-10、T-AOC、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷草转氨酶(ALT)、谷丙转氨酶(AST)和IL-6。结果:(1)血清ALT和AST含量,AL组和E+AL组非常显著高于N组(P<0.01),AL组高于E+AL组(P<0.05);(2)脾脏IL-10含量,AL和E+AL组明显高于N组,E组低于N组,E+AL组高于AL组;AL组和E+AL组血清IL-6含量高于N组(P<0.01),E+AL低于AL组(P<0.05);(3)肝脏T-AOC,AL组、E+AL组低于(P<0.01、P<0.05)、E组高于(P<0.05)N组,AL组低于E+AL组(P<0.05);肝脏SOD,与N组比较,AL组(P<0.05)、E+AL组降低、E组升高,E+AL组高于AL组(P<0.05),MDA含量,与N组相比,E组降低(P<0.05),AL组升高(P<0.01);E+AL组显著性低于AL组(P<0.05)。结论:乙醇在肝脏内代谢过程中会引起机体免疫反应和炎症反应,同时导致肝脏的脂质过氧化损伤;适度的有氧运动干预可提高各实验对象抗氧化能力和抗炎能力,促进机体免疫稳定,减轻肝脏脂质过氧化损伤和炎症损伤,对酒精灌胃所致肝细胞损伤、炎症反应具有一定的缓解作用,提高机体免疫稳定能力,但并不能阻止酒精性肝损伤的发生。更多还原

【Abstract】 Objective: To analyze the changes of liver function,IL-10,IL-6 and T-AOC in mice,intervene by alcoholic and aerobic exercise,to explore its effects on alcoholic liver injury and its mechanism.Methods: 40 mice were randomly divided into control group(N),exercise group(E),alcohol group(AL),exercise plus alcohol group(E + AL),constructing a model of aerobic exercise and alcoholic liver injury.After 10 weeks,taking the serum,liver and spleen,the AST,ALT,IL-10,IL-6,T-AOC,SOD and MDA index were tested.Results:(1) the content of ALT and AST,compared with N group,AL and E + AL group were significantly increased(P < 0.01);group E + AL was significant lower than that in AL group(P < 0.05);(2) the content of IL-10 in spleen lymphocyte,compared with N group,AL and E + AL group are decreased significantly,but E + AL group are increased vs.AL group.The content of serum IL-6,AL and E + AL group were significantly higher than that in N group(P < 0.01),E group was lower,and E + AL was lower than that of AL group(P < 0.05);(3) the level of T-AOC in hepatic tissue,compared with group N,AL and E + AL group were decreased significantly(P < 0.01,P < 0.05),E + AL group was significantly higher than that of AL(P < 0.05);The level of SOD,AL and E + AL group were significantly lower than in group N;E + AL group was raised than AL group(P < 0.05).The content of MDA,compared with that of N group,E group decreased significantly(P < 0.05),AL group was increased significantly(P < 0.01);E + AL group was significantly lower than those in group AL(P < 0.05).Conclusion: Aerobic exercise can improve the ability of anti-oxidation,immunity and inflammation,reduce liver lipid per oxidation and inflammatory injury of liver caused by alcohol,and has a preventive effect on the damage,but can’t prevent the occurring of alcoholic liver injury.更多还原