【摘要】 目的探讨乙肝血清学两种常见模式与相应乙肝核酸定量及两种模式的危险性。方法分别采用酶联免疫吸附法(ELISA法)与实时荧光定量PCR法对60例乙肝患者的血清标志物与相应乙肝核酸定量检测,分别记为模式1与模式2。结果①两种模式阳性率与相应乙肝核酸阳性率差异均具有统计学意义(P<0.05),且模式1乙肝核酸阳性率与乙肝核酸载量对数值均明显大于模式2(P<0.01),但二者阳性检测率无统计学差异(P>0.05);②两种模式下HBsAg、HBeAg、HBcAb组合与HBsAb、HBeAb、HBcAb组合阳性检测率无统计学差异(P>0.05),但两种模式下HBsAg、HBeAb、HBcAb组合阳性检测率具有统计学差异(P<0.05);③模式1HBsAg、HBcAb组合与HBeAb阳性检测率均明显大于模式2(P<0.05),但两种模式下全阴性检测结果无统计学差异(P>0.05);④两种模式下HBsAg、HBeAb、HBcAb组合及HBsAb、HBeAb、HBcAb组合HBV DNA阳性率、HBV DNA含量与HBsAg、HBeAg、HBcAb组合相比,差异均具有统计学意义(P<0.05,P<0.01)。结论实时荧光定量PCR法具有高敏感度与高特异度等特点,在乙肝早期诊断、传染性水平及病毒复制水平等方面具有十分重要的价值;HBV M阳性率与HBV DNA含量具有较好的相关性。更多还原

【Abstract】 Objective To explore the presence of 2 common serological modes of hepatitis B and HBV DNA level and to analyze the corresponding risk of these two modes. Methods Enzyme-linked immunosorbent assay( ELISA) had been applied to detect serum HBV markers and real-time quantitative PCR assay was applied for quantitative detection of nucleic acid in 60 patients with hepatitis B,and serum HBV markers were denoted as Mode 1 and Mode 2. Results ①The positive rates of these two modes were compared with corresponding HBV DNA load,the difference in their positive rates was statistically significant( P < 0. 05),and the positive rate of HBV DNA and HBV DNA load in mode 1 were significantly larger than those of mode 2( P < 0. 01),but the difference in positive detection rate was not significant( P > 0. 05). ②Among these two modes,the difference in positive detection rate in combination of HBsAg,HBeAg and HBcAb and combination of HBsAb,HBeAb and HBcAb was not significant( P > 0. 05),but the difference in positive rate of HBsAg,HBeAb and HBcAb was significant( P < 0. 05). ③In mode 1,the positive detection rate of combination of HBsAg,HBeAg and HBcAb was significantly greater than that of mode 2( P < 0. 05),but the difference in result of full negative test between these two modes was not significant( P > 0. 05). ④Among two modes in combination of HBsAg,HBeAg,HBcAb and combination of HBsAb,HBeAb,HBcAb,the difference in positive rate of HBV DNA and HBV DNA load was statistically significant( P < 0. 05,P < 0. 01). Conclusion Real-time PCR assay has high sensitivity and high specificity,and it is important in early diagnosis of hepatitis B and assessment of level of viral replication. There is good correlation between positive rate of serum HBV markers and HBV DNA load.更多还原