【摘要】 目的:探讨口腔鳞癌中转化生长因子-β1(transforming growth factor-β1,TGF-β1)mRNA的表达及其与临床病理学特征和肿瘤血管生成的关系。方法:应用组织原位杂交和免疫组化检测48例口腔鳞癌组织和10例正常口腔黏膜组织中TGF-β1 mRNA表达,并计数微血管密度(MVD),用SPSS 13.0软件包中的t检验及χ2检验进行统计学分析。结果:TGF-β1mRNA定位于肿瘤细胞胞质,在口腔鳞癌中的表达明显高于正常口腔黏膜组织(P<0.01);TGF-β1 mRNA的表达与口腔鳞癌的浸润深度、淋巴结转移密切相关(P<0.01),而与患者的年龄、性别及病理分级无关;TGF-β1 mRNA的表达与MVD值呈正相关(P<0.01)。结论:TGF-β1 mRNA的高表达可能在口腔鳞癌的浸润和淋巴结转移过程中起重要作用,并与肿瘤血管生成有关。更多还原

【Abstract】 Objective: To investigate the correlation between transforming growth factor-β1(TGF-β1) gene expression and clinicopathologic characteristics in oral squamous cell carcinoma(OSCC).Methods: TGF-β1 mRNA and protein epression in 48 cases of OSCC and 10 cases of normal controls were detected by in-situ hybridization and immunohistochemical staining respectively,microvessel density(MVD) in OSCC was measured.Statistical analysis was performed using t-test and chi-square test by SPSS 13.0.Results: TGF-β1 mRNA and protein were mainly detected in cytoplasm in OSCC cells.The expression of TGF-β1 mRNA and protein were significantly higher in OSCC than in normal oral tissues(P<0.01).Lymph node metastasis and infiltration depth of OSCC cells were closely correlated with TGF-β1 expression(P<0.05),but there was no significant correlation between the positive expression and sex,age of the patients and the differentiation degree of OSCC(P>0.05).TGFF-β1 expression was positively correlated with MVD in OSCC(P<0.01).Conclusion: TGF-β1 overexpression in OSCC may play a potential role in the development,lymph node metastasis and the angiogenesis of OSCC.更多还原