【摘要】 目的:探讨核内原癌基因(nuclear oncogene,MYC)、B细胞淋巴瘤/白血病-2基因(B cell lymphoma/leukemia 2 gene,Bcl-2)、细胞周期蛋白D1(Ccnd1)和细胞角蛋白19(cytokeratin 19,CK19)基因表达水平在乳腺癌诊断和治疗监测中的应用。方法:建立荧光定量聚合酶链反应(FQ-PCR)法,并以GAPDH为内对照测定34名健康女性体检者、55例良性乳腺疾病患者和91例乳腺癌患者外周血中MYC,Ccnd1,Bcl-2和CK19的表达量。结果:MYC,Ccnd1,Bcl-2和CK19表达水平与GAPDH的比值在乳腺癌组显著高于健康女性体检者和良性乳腺疾病患者(P<0.05)。它们在正常对照组和良性乳腺疾病组间差异无统计学意义(P>0.05)。四组GAPDH差异无统计学意义(P>0.05)。MYC,Ccnd1,Bcl-2与CK19联合检测的灵敏度高于单个基因的检测。结论:FQ-PCR技术可以快速定量检测MYC,Ccnd1,Bcl-2和CK19 mRNA,四者联合检测可有效提高对乳腺癌诊断的灵敏度。更多还原

【Abstract】 Objective: To evaluate the clinical application of MYC,Ccnd1,Bcl-2 and CK19 gene in the diagnosis of breast cancer by combined detection. Methods: MYC,Ccnd1,Bcl- 2 and CK19 of 34 health women,55 patients with benign breast disease and 91 patients with breast cancer were detected by FQ- PCR. GAPDH was used as internal control. Results: MYC,Ccnd1,Bcl- 2 and CK19 / GAPDH in breast cancer group were higher than those in health women and patients with benign breast disease groups( P < 0. 05). There was no significant difference in MYC,breast cancer,Bcl- 2 and CK19 / GAPDH between normal controls and benign breast disease group( P > 0. 05). There was no difference in GAPDH among four groups( P > 0. 05). The sensitivity of MYC,Ccnd1,Bcl- 2 and CK19 gene express by combined detection was higher than that of single gene detection. Conclusion: FQ- PCR is a rapid method for quantitating MYC,Ccnd1,Bcl- 2 and CK19. The combined detection can improve the sensitivity of diagnosis in breast cancer.更多还原