【摘要】 目的:通过甲基化特异性PCR研究比较女性散发性基底细胞型乳腺癌和非基底细胞型乳腺癌中ERα基因启动子区甲基化情况。方法:筛选基底细胞型乳腺癌,用甲基化特异性PCR研究60例散发性基底细胞型乳腺癌及108例非基底细胞型乳腺癌的ERα启动子甲基化情况,比较二者ERα基因启动子区甲基化情况。结果:女性散发性基底细胞型乳腺癌组织中ERα基因启动子甲基化发生率为80.0%(48/60),高于其在女性散发性非基底细胞型乳腺癌ERα基因启动子甲基化率43.5%(47/108)(χ2=20.89,P<0.01),二者在ER1与ER5区域的甲基化无统计学差异,但在ER3与ER4区域的甲基化差异有统计学意义。结论:女性散发性基底细胞型乳腺癌组织中ERα基因启动子甲基化发生率明显高于非基底细胞型女性乳腺癌,且基底细胞型乳腺癌ER3与ER4区域的甲基化高于非基底细胞型乳腺癌,其可能与基底细胞型乳腺癌发生发展有关。更多还原

【Abstract】 Objective:To discuss the promoter methylation of estrogen receptor-alpha in sporadic basal-like and non basal-like breast cancer by methylation-specific polymerase chain reaction.Methods:To screen female sporadic basal-like breast cancer,and the methylation of ERα promoter was investigated in genomic DNA of 60 sporadic basal-like breast cancer and 108 cases of non basal-like breast cancer as control by methylation-specific polymerase chain reaction.Results:ERα methylation was observed in 48 of 60(80.0%) sporadic basal-like breast cancer,which was significant higher than in sporadic non basal-like breast cancer(47/108,43.5%)(P<0.01).There was no significancant difference in the methylation status of ER1 region and ER5 region between sporadic basal-like breast cancer and sporadic non basal-like breast cancer.The methylation status of ER3 region and ER4 region in sporadic basal-like breast cancer was higher than in sporadic non basal-like breast cancer.Conclusion:The ERα methylation status in basal-like breast cancer was significantly higher than in sporadic non basal-like breast cancer.The methylation status of ER3 region and ER4 region in sporadic basal-like breast cancer was higher than in sporadic non basal-like breast cancer,maybe it plays an important role in basal-like breast cancer pathogenesis.更多还原