【摘要】 目的:检测胃癌组织中抑癌基因AIF与Calpain-I mRNA水平的表达情况,探讨其在胃癌发生发展中的作用及其临床意义。方法:实时荧光定量PCR法检测64例胃癌组织及其对应的癌旁组织中AIF与Cal-pain-I mRNA表达情况。结果:胃癌组织中AIF与Calpain-I mRNA表达水平较癌旁组织上调,其阳性率分别为84%和75%,差异具有统计学意义(P﹤0.05)。早期胃癌中AIF与Calpain-I mRNA表达水平增高,随着肿瘤恶性程度的增高、临床分期的变晚,逐渐表现出下调趋势。结论:在胃癌组织中,AIF与Calpain-I mR-NA表达水平较癌旁正常组织上调,且与胃癌的临床分期显著相关。更多还原

【Abstract】 Objective:To detect the expression level of AIF and Calpain-I mRNA in gastric cancer and discuss the correlation with occurrence and development of gastric cancer and clinical significance.Methods: Real-time fluorescence quantitative RT-PCR was used to detect AIF and Calpain-I mRNA expression level in 64 cases of gastric cancer and para-cancer normal tissues.Results: The expression level of AIF(84%) and Calpain-I(75%) mRNA in gastric cancer was up-regulated obviously compared to para-cancer normal tissues(P<0.05).Furthermore,the expression level of AIF and Calpain-I mRNA was upper expressed in advanced gastric cancer compared to early gastric cancer(P<0.05).With the higher degree of malignant and clinical stage,it seems to decline gradually.Conclusion: The expression level of AIF and Calpain-I mRNA was over expressed in gastric cancer tissues,and it had relationship with the clinical staging of gastric cancer.更多还原