【摘要】 利用高速逆流色谱对两株昆虫病原真菌蜡蚧霉发酵液的乙酸乙酯粗提物进行分离纯化,GC/MS对分离物进行检测.结果表明:利用高速逆流色谱经两相溶剂系统:正己烷-乙酸乙酯-甲醇-水(4∶5∶4∶5)均可从两菌株代谢产物中分离纯化得到邻苯二甲酸二丁酯,研究发现蜡蚧霉代谢产物中存在天然的邻苯二甲酸二丁酯.更多还原

【Abstract】 High-speed counter-current chromatography(HSCCC) was applied to the isolation and purification of secondary metabolites from Lecanicilliurn lecanii(Zimmermann),and the compounds were identified by GC-MS.The result showed that dibutyl phthalate first found in natural metabolites from L.lecanii were obtained by using HSCCC with opportune two-phase solvent system composed of hexane-ethyl acetate-methanol-water(4︰5︰4︰5,V/V).更多还原