Objective To express porcine interleukin-2(poIL-2)in prokaryotic cells,purify the expressed product and determine its bioactivity. Methods The poIL-2 gene was amplified by PCR using the genomic RNA extracted from porcine peripheral blood lymphocytes as a template,and inserted into prokaryotic expression vector pBV220. The constructed recombinant plasmid pBV220 / IL-2 was transformed to competent E. coli DH5α,and the recombinants were screened with LB plate containing Amp,then cultured in LB medium. Viable c...
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